Index of /public/revio/2023Q3/ZymoTrumatrix
Name Last modified Size Description
Parent Directory -
README.txt 2023-07-23 15:37 727
m84028_230113_224423_s1.hifi_reads.bam 2023-07-19 12:16 32G
m84028_230113_231547_s2.hifi_reads.bam 2023-07-19 12:20 31G
OVERVIEW
This directory includes metagenomic sequencing datasets from the
Revio system for the ZymoBIOMICS Fecal Reference with TruMatrix Technology
(Zymo Research, D6323), with one Revio SMRT Cell per sample replicate.
MOVIE
m84028_230113_224423_s1 Revio replicate 1; 7.6 kb mean read length; QV46 median read quality
m84028_230113_231547_s2 Revio replicate 2; 7.7 kb mean read length; QV46 median read quality
METHODS
LIBRARY PREP SMRTbell prep kit 3.0
SIZE SELECTION Cleanup with 3.7X v/v of diluted 35% AMPure PB beads to remove fragments <3kb
SEQUENCING Revio system, 24 hour movie
ANALYSIS Generate HiFi reads with 5mC methylation calls on the Revio system