Index of /public/revio/2023Q3/ZymoTrumatrix

Icon  Name                                   Last modified      Size  Description
[PARENTDIR] Parent Directory                                            -   
[   ] m84028_230113_224423_s1.hifi_reads.bam 2023-07-19 12:16   32G  
[   ] m84028_230113_231547_s2.hifi_reads.bam 2023-07-19 12:20   31G  
[TXT] README.txt                             2023-07-23 15:37  727   

OVERVIEW
    This directory includes metagenomic sequencing datasets from the
    Revio system for the ZymoBIOMICS Fecal Reference with TruMatrix Technology
    (Zymo Research, D6323), with one Revio SMRT Cell per sample replicate.

MOVIE
    m84028_230113_224423_s1    Revio replicate 1; 7.6 kb mean read length; QV46 median read quality
    m84028_230113_231547_s2    Revio replicate 2; 7.7 kb mean read length; QV46 median read quality

METHODS
    LIBRARY PREP     SMRTbell prep kit 3.0
    SIZE SELECTION   Cleanup with 3.7X v/v of diluted 35% AMPure PB beads to remove fragments <3kb
    SEQUENCING       Revio system, 24 hour movie
    ANALYSIS         Generate HiFi reads with 5mC methylation calls on the Revio system