Index of /public/sequelii/2023Q3/ZymoTrumatrix

 Name                                 Last modified      Size  Description
 Parent Directory                                          -   
 README.txt                           2023-07-23 15:37  1.2K  
 m64358e_211009_000148.hifi_reads.bam 2023-07-19 11:57   16G  
 m64358e_211010_105543.hifi_reads.bam 2023-07-19 11:58   16G  
 m64402e_220124_225429.hifi_reads.bam 2023-07-19 11:59  8.9G  
 m64402e_220126_094615.hifi_reads.bam 2023-07-19 12:01   11G  
 m64402e_220127_203951.hifi_reads.bam 2023-07-19 12:01  8.1G  
 m64402e_220129_073242.hifi_reads.bam 2023-07-19 12:03  9.6G  

OVERVIEW
    This directory includes metagenomic sequencing datasets from the
    Sequel IIe system for the ZymoBIOMICS Fecal Reference with TruMatrix Technology
    (Zymo Research, D6323), with one SMRT Cell 8M per sample replicate.

MOVIE
    m64358e_211009_000148      Sequel IIe, TPK 2.0; 8.7 kb mean read length, QV40 median read quality
    m64358e_211010_105543      Sequel IIe, TPK 2.0; 8.3 kb mean read length, QV42 median read quality
    m64402e_220124_225429      Sequel IIe, TPK 2.0; 6.3 kb mean read length, QV39 median read quality
    m64402e_220126_094615      Sequel IIe, SPK 3.0; 6.1 kb mean read length, QV46 median read quality
    m64402e_220127_203951      Sequel IIe, SPK 3.0; 5.8 kb mean read length, QV44 median read quality
    m64402e_220129_073242      Sequel IIe, SPK 3.0; 6.3 kb mean read length, QV48 median read quality

METHODS
    LIBRARY PREP     SMRTbell Express Template Prep Kit 2.0 (TPK), or SMRTbell prep
                     kit 3.0 (SPK)
    SIZE SELECTION   Cleanup with 3.7X v/v of diluted 35% AMPure PB beads to remove fragments <3kb
    SEQUENCING       Sequel IIe system, 30 hour movie
    ANALYSIS         Generate HiFi reads using ccs v6.2