Index of /public/revio/2024Q4/WGS/GIAB_trio

 Name                      Last modified      Size  Description
 Parent Directory                               -   
 HG004/                    2024-11-18 21:05    -   
 HG003/                    2024-11-18 21:05    -   
 HG002_rep2/               2024-11-18 21:05    -   
 HG002_rep1/               2024-11-18 21:05    -   
 README.txt                2024-10-29 14:30  674   

OVERVIEW
    This directory includes human whole genome sequencing datasets from the
    Revio system with SPRQ chemistry for the Genome in a Bottle trio HG002+HG003+HG004, with one
    Revio SMRT Cell per sample replicate.


DIRECTORIES
    HG002-rep1      HG002 replicate 1
    HG002-rep2      HG002 replicate 2
    HG003-rep1      HG003 replicate 1
    HG004-rep1      HG004 replicate 1


METHODS
    SHEARING         Pipette-tip shearing on hte Hamilton NGS STAR MOA 96 system
    LIBRARY PREP     SMRTbell prep kit 3.0
    SEQUENCING       Revio system, SPRQ chemistry, 24 hour movie
    ANALYSIS         Generate HiFi reads with methylation calls on the Revio system