OVERVIEW This directory includes human whole genome sequencing datasets from the Revio system with SPRQ chemistry for the Genome in a Bottle trio HG002+HG003+HG004, with one Revio SMRT Cell per sample replicate. DIRECTORIES HG002-rep1 HG002 replicate 1 HG002-rep2 HG002 replicate 2 HG003-rep1 HG003 replicate 1 HG004-rep1 HG004 replicate 1 METHODS SHEARING Pipette-tip shearing on hte Hamilton NGS STAR MOA 96 system LIBRARY PREP SMRTbell prep kit 3.0 SEQUENCING Revio system, SPRQ chemistry, 24 hour movie ANALYSIS Generate HiFi reads with methylation calls on the Revio system