Index of /public/revio/2024Q4/WGS/GIAB_trio
Name Last modified Size Description
Parent Directory -
HG002_rep1/ 2024-11-18 21:05 -
HG002_rep2/ 2024-11-18 21:05 -
HG003/ 2024-11-18 21:05 -
HG004/ 2024-11-18 21:05 -
README.txt 2024-10-29 14:30 674
OVERVIEW
This directory includes human whole genome sequencing datasets from the
Revio system with SPRQ chemistry for the Genome in a Bottle trio HG002+HG003+HG004, with one
Revio SMRT Cell per sample replicate.
DIRECTORIES
HG002-rep1 HG002 replicate 1
HG002-rep2 HG002 replicate 2
HG003-rep1 HG003 replicate 1
HG004-rep1 HG004 replicate 1
METHODS
SHEARING Pipette-tip shearing on hte Hamilton NGS STAR MOA 96 system
LIBRARY PREP SMRTbell prep kit 3.0
SEQUENCING Revio system, SPRQ chemistry, 24 hour movie
ANALYSIS Generate HiFi reads with methylation calls on the Revio system