Index of /public/2026Q2/HG002-SPRQ-Nx

 Name                    Last modified      Size  Description
 Parent Directory                             -   
 Use3/                   2026-06-15 23:44    -   
 Use2/                   2026-06-15 23:43    -   
 Use1/                   2026-06-15 23:43    -   
 README.txt              2026-06-15 23:59  1.0K  

OVERVIEW
    This directory includes human HG002 WGS datasets produced
    with SPRQ-Nx multi-use chemistry and three acquisitions
    per SMRT Cell.  It utilized the release instrument software
    which includes 5mC, 5hmC, and 6mA callers.


METHODS
    SHEARING      Pipette-tip shearing on Hamilton NGS STAR MOA 96 system
    LIBRARY PREP  HiFi prep kit 96 on Hamilton NGS STAR MOA 96 system
    SEQUENCING    Revio system, 24-hour movies
                  SPRQ-Nx chemistry
    ANALYSIS      On-instrument HiFi base and methylation (5mCpG, 5hmCpG, 6mA) calling
                  Secondary analysis with HiFi WGS variant pipeline

RESULTS
    SMRT Cell  Use  Movie                       Yield  Reads   Length
    ---------  ---  -----------------------  --------  -----  -------
    EA314518     1  m84031_260504_231011_s1  144.6 Gb   8.9M  16.2 kb
    EA314518     2  m84031_260506_033729_s1  143.7 Gb   8.2M  17.4 kb
    EA314518     3  m84031_260507_084733_s1  127.2 Gb   7.1M  17.9 kb