OVERVIEW This directory includes human HG002 WGS datasets produced with SPRQ-Nx multi-use chemistry and three acquisitions per SMRT Cell. It utilized the release instrument software which includes 5mC, 5hmC, and 6mA callers. METHODS SHEARING Pipette-tip shearing on Hamilton NGS STAR MOA 96 system LIBRARY PREP HiFi prep kit 96 on Hamilton NGS STAR MOA 96 system SEQUENCING Revio system, 24-hour movies SPRQ-Nx chemistry ANALYSIS On-instrument HiFi base and methylation (5mCpG, 5hmCpG, 6mA) calling Secondary analysis with HiFi WGS variant pipeline RESULTS SMRT Cell Use Movie Yield Reads Length --------- --- ----------------------- -------- ----- ------- EA314518 1 m84031_260504_231011_s1 144.6 Gb 8.9M 16.2 kb EA314518 2 m84031_260506_033729_s1 143.7 Gb 8.2M 17.4 kb EA314518 3 m84031_260507_084733_s1 127.2 Gb 7.1M 17.9 kb