Index of /public/2025Q2/HG008

 Name                    Last modified      Size  Description
 Parent Directory                             -   
 HG008-N-P/              2025-03-31 21:01    -   
 HG008-T/                2025-03-31 21:01    -   
 README.txt              2025-03-31 21:08  712   

OVERVIEW
    This directory contains human whole genome sequencing datasets for the Genome in a Bottle
    tumor/normal reference sample: HG008-N-P (normal pancreatic) and HG008-T (tumor). Sequencing
    was performed using both the Revio system with SPRQ chemistry and the Vega system.


METHODS
    SAMPLES          HG008-N-P and HG008-T was provided by Genome in a Bottle (Zook)
    SHEARING         Pipette-tip shearing on the Hamilton NGS STAR MOA 96 system
    LIBRARY PREP     SMRTbell prep kit 3.0
    SEQUENCING       Revio system, SPRQ chemistry, 24-hour movie
                     Vega system, 24-hour movie
    ANALYSIS         Generate HiFi reads with methylation calls on the Revio or Vega system