OVERVIEW This directory contains human whole genome sequencing datasets for the Genome in a Bottle tumor/normal reference sample: HG008-N-P (normal pancreatic) and HG008-T (tumor). Sequencing was performed using both the Revio system with SPRQ chemistry and the Vega system. METHODS SAMPLES HG008-N-P and HG008-T was provided by Genome in a Bottle (Zook) SHEARING Pipette-tip shearing on the Hamilton NGS STAR MOA 96 system LIBRARY PREP SMRTbell prep kit 3.0 SEQUENCING Revio system, SPRQ chemistry, 24-hour movie Vega system, 24-hour movie ANALYSIS Generate HiFi reads with methylation calls on the Revio or Vega system