Index of /public/2025Q2/HG008
OVERVIEW
This directory contains human whole genome sequencing datasets for the Genome in a Bottle
tumor/normal reference sample: HG008-N-P (normal pancreatic) and HG008-T (tumor). Sequencing
was performed using both the Revio system with SPRQ chemistry and the Vega system.
METHODS
SAMPLES HG008-N-P and HG008-T was provided by Genome in a Bottle (Zook)
SHEARING Pipette-tip shearing on the Hamilton NGS STAR MOA 96 system
LIBRARY PREP SMRTbell prep kit 3.0
SEQUENCING Revio system, SPRQ chemistry, 24-hour movie
Vega system, 24-hour movie
ANALYSIS Generate HiFi reads with methylation calls on the Revio or Vega system