Index of /public/revio/2024Q4/WGS/HG002_fiberseq

 Name                      Last modified      Size  Description
 Parent Directory                               -   
 Fiber-seq_hWGS_HG002/     2024-11-18 21:05    -   
 README.txt                2024-10-26 13:17  700   

OVERVIEW
    This directory includes fiber-seq human whole genome sequencing datasets from the
    Revio system with SPRQ chemistry for the GIAB HG002 control sample.


DIRECTORIES
    Fiberseq_hWGS_HG002


METHODS
    Fiber-seq processing   Performed as desribed in Stergachis AB, et al. 2020. Single molecule regulatory architectures captured by chromatin fiber sequencing. Science 368:1449–1454.
    SHEARING               Pipette-tip shearing on hte Hamilton NGS STAR MOA 96 system
    LIBRARY PREP           SMRTbell prep kit 3.0
    SEQUENCING             Revio system, SPRQ chemistry, 24 hour movie
    ANALYSIS               Generate HiFi reads with methylation calls on the Revio system