Index of /public/revio/2024Q4/WGS/COLO829

 Name                      Last modified      Size  Description
 Parent Directory                               -   
 COLO829BL/                2024-10-26 12:11    -   
 COLO829/                  2024-10-26 12:13    -   
 README.txt                2024-10-29 14:29  629   

OVERVIEW
    This directory includes human whole genome sequencing datasets generated
    on the Revio system with SPRQ chemistry for the COLO829 (tumor) and COLO829-BL (matched normal) cell lines.


METHODS
    SAMPLES          ATCC cells CRL-1974 for COLO829
                     ATCC cells CRL-1980 for COLO829-BL
    EXTRACTION       Nanobind CBB kit
    SHEARING         Pipette-tip shearing on hte Hamilton NGS STAR MOA 96 system
    LIBRARY PREP     SMRTbell prep kit 3.0
    SEQUENCING       Revio system, SPRQ chemistry, 24 hour movie
    ANALYSIS         Generate HiFi reads with methylation calls on the Revio system