Index of /public/revio/2024Q4/WGS/COLO829
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COLO829BL/ 2024-10-26 12:11 -
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README.txt 2024-10-29 14:29 629
OVERVIEW
This directory includes human whole genome sequencing datasets generated
on the Revio system with SPRQ chemistry for the COLO829 (tumor) and COLO829-BL (matched normal) cell lines.
METHODS
SAMPLES ATCC cells CRL-1974 for COLO829
ATCC cells CRL-1980 for COLO829-BL
EXTRACTION Nanobind CBB kit
SHEARING Pipette-tip shearing on hte Hamilton NGS STAR MOA 96 system
LIBRARY PREP SMRTbell prep kit 3.0
SEQUENCING Revio system, SPRQ chemistry, 24 hour movie
ANALYSIS Generate HiFi reads with methylation calls on the Revio system