Index of /public/revio/2023Q2/HCC1395

Name	Last modified (UTC)	Size
Parent Directory		-
HCC1395-BL/		-
HCC1395/		-
README.txt	2023-07-07 05:50	839

OVERVIEW
    This directory includes human whole genome sequencing datasets generated
    on the Revio system for the HCC1395 (tumor) and HCC1395-BL (matched normal) cell lines.


METHODS
    SAMPLES          ATCC genomic DNA CRL-2324D for HCC1395
                     ATCC genomic DNA CRL-2325D for HCC1395-BL
    SHEARING         Megaruptor 3 to target size of 15-20 kb
    LIBRARY PREP     SMRTbell prep kit 3.0
    SEQUENCING       Revio system, 24 hour movie
    ANALYSIS         Generate HiFi reads with methylation calls on the Revio system
                     Align to GRCh38_no_alt_analysis_set with pbmm2 v1.10.0


DATA
    Sample         Yield        Reads   Read length
    ----------  --------  -----------  ------------
    HCC1395-BL  128.0 Gb    7,591,952     16,863 bp
    HCC1395     180.9 Gb   12,662,343     14,285 bp