Index of /public/revio/2023Q2/COLO829

Icon  Name                    Last modified      Size  Description
[PARENTDIR] Parent Directory - [DIR] COLO829-BL/ 2023-04-11 16:30 - [DIR] COLO829/ 2023-04-11 16:30 - [TXT] README.txt 2023-04-09 22:38 679
    This directory includes human whole genome sequencing datasets generated
    on the Revio system for the COLO829 (tumor) and COLO829-BL (matched normal) cell lines.

    SAMPLES          ATCC cells CRL-1974 for COLO829
                     ATCC cells CRL-1980 for COLO829-BL
    EXTRACTION       Nanobind CBB kit
    SHEARING         Megaruptor 3 to target size of 15-20 kb
    LIBRARY PREP     SMRTbell prep kit 3.0 + SMRTbell size selection wash
    SEQUENCING       Revio system, 24 hour movie
    ANALYSIS         Generate HiFi reads with methylation calls on the Revio system
                     Align to GRCh38_no_alt_analysis_set with pbmm2 v1.10.0