Index of /public/revio/2023Q2/COLO829

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COLO829/-
README.txt2023-04-10 05:38679
OVERVIEW
    This directory includes human whole genome sequencing datasets generated
    on the Revio system for the COLO829 (tumor) and COLO829-BL (matched normal) cell lines.


METHODS
    SAMPLES          ATCC cells CRL-1974 for COLO829
                     ATCC cells CRL-1980 for COLO829-BL
    EXTRACTION       Nanobind CBB kit
    SHEARING         Megaruptor 3 to target size of 15-20 kb
    LIBRARY PREP     SMRTbell prep kit 3.0 + SMRTbell size selection wash
    SEQUENCING       Revio system, 24 hour movie
    ANALYSIS         Generate HiFi reads with methylation calls on the Revio system
                     Align to GRCh38_no_alt_analysis_set with pbmm2 v1.10.0