Index of /public/revio/2023Q2/COLO829

 Name                    Last modified      Size  Description
 Parent Directory                             -   
 COLO829/                2023-04-11 16:30    -   
 COLO829-BL/             2023-04-11 16:30    -   
 README.txt              2023-04-09 22:38  679   

OVERVIEW
    This directory includes human whole genome sequencing datasets generated
    on the Revio system for the COLO829 (tumor) and COLO829-BL (matched normal) cell lines.


METHODS
    SAMPLES          ATCC cells CRL-1974 for COLO829
                     ATCC cells CRL-1980 for COLO829-BL
    EXTRACTION       Nanobind CBB kit
    SHEARING         Megaruptor 3 to target size of 15-20 kb
    LIBRARY PREP     SMRTbell prep kit 3.0 + SMRTbell size selection wash
    SEQUENCING       Revio system, 24 hour movie
    ANALYSIS         Generate HiFi reads with methylation calls on the Revio system
                     Align to GRCh38_no_alt_analysis_set with pbmm2 v1.10.0