Index of /public/revio/2023Q1

Icon  Name                    Last modified      Size  Description
[PARENTDIR] Parent Directory                             -   
[DIR] ladybug-rep1/           2023-01-10 09:12    -   
[DIR] ladybug-rep2/           2023-01-10 09:12    -   
[DIR] maize-B73-rep1/         2023-02-25 23:42    -   
[DIR] mistletoe-rep1/         2023-01-10 09:12    -   
[DIR] mistletoe-rep2/         2023-01-10 09:12    -   
[DIR] mouse-rep1/             2023-01-10 09:12    -   
[DIR] mouse-rep2/             2023-01-10 09:12    -   
[DIR] oak-rep1/               2023-01-10 09:12    -   
[DIR] oak-rep2/               2023-01-10 09:12    -   
[DIR] revio-file-layout/      2023-07-24 07:23    -   
[TXT] README.txt              2023-02-25 23:42  2.4K  

OVERVIEW
    This directory includes example datasets from the Revio system.


PLANT+ANIMAL WHOLE GENOME SEQUENCING
    SAMPLES
        Samples were provided by the Tree of Life Programme at the Sanger Institute
        and other collaborators.

        Common name    Species name         Barcodes
        ===========    =================    ===============
        Mouse          Mus musculus         bc2088
        Ladybug        Adalia bipunctata    bc2094
        Oak            Quercus robur        bc2086
        Mistletoe      Viscum album         bc2087, bc2093
        Maize B73      Zea mays             bc2015, bc2016, bc2023, bc2032


    METHODS
        SHEARING         Megaruptor 3 to target size of 15-20 kb (mouse, ladybug, oak, mistletoe)
                         Pipette shearing to target size of 15-20 kb (maize)
        LIBRARY PREP     SMRTbell prep kit 3.0
                         SMRTbell barcoded adapter plate 3.0
                         SMRTbell size selection wash
        SEQUENCING       Revio system, 24 hour movie
        ANALYSIS         Generate HiFi reads with methylation calls on the Revio system
                         Demultiplex barcodes with lima


    RUNS
                                    Yield                              Yield
        Movie                     (total)    Sample     Replicate   (sample)
        =======================   =======    ========   =========   ========
        m84005_221008_003539_s1   90.5 Gb    Mouse          1        69.6 Gb
                                             Ladybug        1        20.9 Gb
        -----------------------   -------    --------   ---------   --------
        m84006_221008_010259_s2   91.2 Gb    Mouse          2        70.4 Gb
                                             Ladybug        2        20.8 Gb
        -----------------------   -------    --------   ---------   --------
        m84005_221008_010657_s2   92.6 Gb    Oak            1        41.0 Gb
                                             Mistletoe      1        51.6 Gb
        -----------------------   -------    --------   ---------   --------
        m84006_221008_003137_s1   98.1 Gb    Oak            2        45.3 Gb
                                             Mistletoe      2        52.8 Gb
        -----------------------   -------    --------   ---------   --------
        m84006_221229_002525_s1   70.3 Gb    Maize          1        70.3 Gb