>|> 20220329 04:25:45.569 -|- INFO -|- LaaRunner -|- 0x7facab005740|| -|- Welcome to pbaa. >|> 20220329 04:25:45.572 -|- INFO -|- GraphProcessing -|- 0x7facab005740|| -|- 1 sequence entries. >|> 20220329 04:25:45.572 -|- INFO -|- GraphProcessing -|- 0x7facab005740|| -|- 4507 nodes before filtering. >|> 20220329 04:25:45.572 -|- INFO -|- GraphProcessing -|- 0x7facab005740|| -|- 4507 nodes after filtering. >|> 20220329 04:25:45.572 -|- INFO -|- GetGuideInfo -|- 0x7facab005740|| -|- Processing 1 guide loci. >|> 20220329 04:25:45.573 -|- INFO -|- GetGuideInfo -|- 0x7facab005740|| -|- There are 4507 unique kmers within guide sequence(s). >|> 20220329 04:25:45.773 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/1507892/ccs has no guide hits, skipping read. >|> 20220329 04:25:45.838 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/1837837/ccs has no guide hits, skipping read. >|> 20220329 04:25:45.967 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/3213708/ccs has no guide hits, skipping read. >|> 20220329 04:25:46.093 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/5047512/ccs has no guide hits, skipping read. >|> 20220329 04:25:46.282 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/7932383/ccs has no guide hits, skipping read. >|> 20220329 04:25:46.429 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/11995915/ccs has no guide hits, skipping read. >|> 20220329 04:25:46.604 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/17172584/ccs has no guide hits, skipping read. >|> 20220329 04:25:46.776 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/20251524/ccs has no guide hits, skipping read. >|> 20220329 04:25:46.928 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/22217468/ccs has no guide hits, skipping read. >|> 20220329 04:25:47.115 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/24971834/ccs has no guide hits, skipping read. >|> 20220329 04:25:47.473 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/32835954/ccs has no guide hits, skipping read. >|> 20220329 04:25:47.655 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/35653276/ccs has no guide hits, skipping read. >|> 20220329 04:25:47.693 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/37030259/ccs has no guide hits, skipping read. >|> 20220329 04:25:47.696 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/37161451/ccs has no guide hits, skipping read. >|> 20220329 04:25:47.915 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/41092292/ccs has no guide hits, skipping read. >|> 20220329 04:25:48.326 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/48694456/ccs has no guide hits, skipping read. >|> 20220329 04:25:48.827 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/53018735/ccs has no guide hits, skipping read. >|> 20220329 04:25:48.828 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/53084318/ccs has no guide hits, skipping read. >|> 20220329 04:25:48.879 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/54133131/ccs has no guide hits, skipping read. >|> 20220329 04:25:49.133 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/56428232/ccs has no guide hits, skipping read. >|> 20220329 04:25:49.220 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/57999390/ccs has no guide hits, skipping read. >|> 20220329 04:25:49.595 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/61868862/ccs has no guide hits, skipping read. >|> 20220329 04:25:49.664 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/62784576/ccs has no guide hits, skipping read. >|> 20220329 04:25:49.671 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/63048198/ccs has no guide hits, skipping read. >|> 20220329 04:25:50.451 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/74320207/ccs has no guide hits, skipping read. >|> 20220329 04:25:50.610 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/77596595/ccs has no guide hits, skipping read. >|> 20220329 04:25:50.755 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/81332607/ccs has no guide hits, skipping read. >|> 20220329 04:25:51.267 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/90767423/ccs has no guide hits, skipping read. >|> 20220329 04:25:51.269 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/90835611/ccs has no guide hits, skipping read. >|> 20220329 04:25:51.677 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/95289575/ccs has no guide hits, skipping read. >|> 20220329 04:25:51.679 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/95291852/ccs has no guide hits, skipping read. >|> 20220329 04:25:51.788 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/97323005/ccs has no guide hits, skipping read. >|> 20220329 04:25:51.989 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/101386662/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.060 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/102631989/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.288 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/107020684/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.298 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/107414487/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.548 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/111479066/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.683 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/114099458/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.880 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/116852643/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.993 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/119081428/ccs has no guide hits, skipping read. >|> 20220329 04:25:53.050 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/120980426/ccs has no guide hits, skipping read. >|> 20220329 04:25:53.117 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/121637563/ccs has no guide hits, skipping read. >|> 20220329 04:25:53.549 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/128516292/ccs has no guide hits, skipping read. >|> 20220329 04:25:53.851 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/130810708/ccs has no guide hits, skipping read. >|> 20220329 04:25:54.709 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/142869586/ccs has no guide hits, skipping read. >|> 20220329 04:25:54.778 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/145295971/ccs has no guide hits, skipping read. >|> 20220329 04:25:54.858 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/147325066/ccs has no guide hits, skipping read. >|> 20220329 04:25:54.877 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/148113941/ccs has no guide hits, skipping read. >|> 20220329 04:25:54.911 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/149228027/ccs has no guide hits, skipping read. >|> 20220329 04:25:55.020 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/152767067/ccs has no guide hits, skipping read. >|> 20220329 04:25:55.034 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/153420175/ccs has no guide hits, skipping read. >|> 20220329 04:25:55.084 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/155519072/ccs has no guide hits, skipping read. >|> 20220329 04:25:55.627 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/164758508/ccs has no guide hits, skipping read. >|> 20220329 04:25:55.637 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/165151887/ccs has no guide hits, skipping read. >|> 20220329 04:25:55.682 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/165808983/ccs has no guide hits, skipping read. >|> 20220329 04:25:56.080 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/172098702/ccs has no guide hits, skipping read. >|> 20220329 04:25:56.312 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/174522733/ccs has no guide hits, skipping read. >|> 20220329 04:25:56.488 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/176884053/ccs has no guide hits, skipping read. >|> 20220329 04:25:56.722 -|- WARN -|- operator() -|- 0x7facab005740|| -|- m64013e_210223_231140/179964058/ccs has no guide hits, skipping read. >|> 20220329 04:26:14.312 -|- WARN -|- BuildKFG -|- 0x7facab005740|| -|- read: m64013e_210223_231140/108463853/ccs rounded length: 17300 skipped > 100 bp. >|> 20220329 04:26:20.345 -|- WARN -|- BuildKFG -|- 0x7facab005740|| -|- read: m64013e_210223_231140/118884681/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:26:21.325 -|- WARN -|- BuildKFG -|- 0x7facab005740|| -|- read: m64013e_210223_231140/121045578/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:26:24.878 -|- WARN -|- BuildKFG -|- 0x7facab005740|| -|- read: m64013e_210223_231140/128714757/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:26:27.508 -|- WARN -|- BuildKFG -|- 0x7facab005740|| -|- read: m64013e_210223_231140/132712926/ccs QV: 19.9909 skipped < Q20. >|> 20220329 04:26:32.134 -|- WARN -|- BuildKFG -|- 0x7facab005740|| -|- read: m64013e_210223_231140/140511377/ccs QV: 19.9991 skipped < Q20. >|> 20220329 04:26:37.903 -|- WARN -|- BuildKFG -|- 0x7facab005740|| -|- read: m64013e_210223_231140/149553921/ccs QV: 19.9885 skipped < Q20. >|> 20220329 04:26:40.272 -|- WARN -|- BuildKFG -|- 0x7facab005740|| -|- read: m64013e_210223_231140/153684349/ccs QV: 19.9969 skipped < Q20. >|> 20220329 04:26:40.994 -|- WARN -|- BuildKFG -|- 0x7facab005740|| -|- read: m64013e_210223_231140/154993079/ccs QV: 19.9902 skipped < Q20. >|> 20220329 04:26:41.659 -|- WARN -|- BuildKFG -|- 0x7facab005740|| -|- read: m64013e_210223_231140/156239645/ccs QV: 19.9836 skipped < Q20. >|> 20220329 04:26:45.308 -|- WARN -|- BuildKFG -|- 0x7facab005740|| -|- read: m64013e_210223_231140/162793830/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:26:47.130 -|- WARN -|- BuildKFG -|- 0x7facab005740|| -|- read: m64013e_210223_231140/166397085/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:26:55.631 -|- WARN -|- BuildKFG -|- 0x7facab005740|| -|- read: m64013e_210223_231140/2097674/ccs QV: 19.9914 skipped < Q20. >|> 20220329 04:26:58.145 -|- WARN -|- BuildKFG -|- 0x7facab005740|| -|- read: m64013e_210223_231140/26019721/ccs QV: 19.9917 skipped < Q20. >|> 20220329 04:27:13.687 -|- WARN -|- BuildKFG -|- 0x7facab005740|| -|- read: m64013e_210223_231140/5178125/ccs QV: 19.9934 skipped < Q20. >|> 20220329 04:27:23.974 -|- WARN -|- BuildKFG -|- 0x7facab005740|| -|- read: m64013e_210223_231140/69730747/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:27:34.745 -|- WARN -|- BuildKFG -|- 0x7facab005740|| -|- read: m64013e_210223_231140/86901665/ccs QV: 19.9941 skipped < Q20. >|> 20220329 04:27:38.822 -|- WARN -|- BuildKFG -|- 0x7facab005740|| -|- read: m64013e_210223_231140/94374508/ccs QV: 19.9927 skipped < Q20. >|> 20220329 04:27:39.115 -|- WARN -|- BuildKFG -|- 0x7facab005740|| -|- read: m64013e_210223_231140/95029435/ccs QV: 19.9969 skipped < Q20. >|> 20220329 04:29:16.434 -|- INFO -|- RunAlignments -|- 0x7facab005740|| -|- Finished 743672 valid pairwise alignments in 86197 ms >|> 20220329 04:30:51.662 -|- INFO -|- ChimeraDetection -|- 0x7facab005740|| -|- Running UCHIME algorithm. >|> 20220329 04:30:51.769 -|- INFO -|- LaaRunner -|- 0x7facab005740|| -|- Finished without error. >|> 20220329 04:30:51.769 -|- INFO -|- LaaRunner -|- 0x7facab005740|| -|- Real time : 5m 6s >|> 20220329 04:30:51.769 -|- INFO -|- LaaRunner -|- 0x7facab005740|| -|- CPU time : 29m 49s >|> 20220329 04:30:51.769 -|- INFO -|- LaaRunner -|- 0x7facab005740|| -|- Peak RSS : 17.676 GB