>|> 20220329 04:25:43.022 -|- INFO -|- LaaRunner -|- 0x7fb676472740|| -|- Welcome to pbaa. >|> 20220329 04:25:43.032 -|- INFO -|- GraphProcessing -|- 0x7fb676472740|| -|- 1 sequence entries. >|> 20220329 04:25:43.032 -|- INFO -|- GraphProcessing -|- 0x7fb676472740|| -|- 4507 nodes before filtering. >|> 20220329 04:25:43.033 -|- INFO -|- GraphProcessing -|- 0x7fb676472740|| -|- 4507 nodes after filtering. >|> 20220329 04:25:43.033 -|- INFO -|- GetGuideInfo -|- 0x7fb676472740|| -|- Processing 1 guide loci. >|> 20220329 04:25:43.033 -|- INFO -|- GetGuideInfo -|- 0x7fb676472740|| -|- There are 4507 unique kmers within guide sequence(s). >|> 20220329 04:25:43.139 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/1639366/ccs has no guide hits, skipping read. >|> 20220329 04:25:43.225 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/3672821/ccs has no guide hits, skipping read. >|> 20220329 04:25:43.238 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/4000215/ccs has no guide hits, skipping read. >|> 20220329 04:25:43.363 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/6947969/ccs has no guide hits, skipping read. >|> 20220329 04:25:43.380 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/7471816/ccs has no guide hits, skipping read. >|> 20220329 04:25:43.385 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/7538917/ccs has no guide hits, skipping read. >|> 20220329 04:25:43.497 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/8848438/ccs has no guide hits, skipping read. >|> 20220329 04:25:43.503 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/8980662/ccs has no guide hits, skipping read. >|> 20220329 04:25:43.688 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/11142972/ccs has no guide hits, skipping read. >|> 20220329 04:25:43.989 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/16188895/ccs has no guide hits, skipping read. >|> 20220329 04:25:44.036 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/17368796/ccs has no guide hits, skipping read. >|> 20220329 04:25:44.049 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/17697228/ccs has no guide hits, skipping read. >|> 20220329 04:25:44.136 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/18942686/ccs has no guide hits, skipping read. >|> 20220329 04:25:44.736 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/25494275/ccs has no guide hits, skipping read. >|> 20220329 04:25:44.866 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/27330599/ccs has no guide hits, skipping read. >|> 20220329 04:25:45.010 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/30934501/ccs has no guide hits, skipping read. >|> 20220329 04:25:45.014 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/31000514/ccs has no guide hits, skipping read. >|> 20220329 04:25:45.043 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/31655577/ccs has no guide hits, skipping read. >|> 20220329 04:25:45.064 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/32115499/ccs has no guide hits, skipping read. >|> 20220329 04:25:45.116 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/33033029/ccs has no guide hits, skipping read. >|> 20220329 04:25:45.145 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/33818388/ccs has no guide hits, skipping read. >|> 20220329 04:25:45.149 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/33950074/ccs has no guide hits, skipping read. >|> 20220329 04:25:45.379 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/37552227/ccs has no guide hits, skipping read. >|> 20220329 04:25:45.380 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/37553466/ccs has no guide hits, skipping read. >|> 20220329 04:25:45.392 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/37815257/ccs has no guide hits, skipping read. >|> 20220329 04:25:45.437 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/38603038/ccs has no guide hits, skipping read. >|> 20220329 04:25:45.461 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/39191026/ccs has no guide hits, skipping read. >|> 20220329 04:25:45.559 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/40894972/ccs has no guide hits, skipping read. >|> 20220329 04:25:46.264 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/48366291/ccs has no guide hits, skipping read. >|> 20220329 04:25:46.348 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/50331828/ccs has no guide hits, skipping read. >|> 20220329 04:25:46.350 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/50333955/ccs has no guide hits, skipping read. >|> 20220329 04:25:46.505 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/52038147/ccs has no guide hits, skipping read. >|> 20220329 04:25:46.687 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/55576187/ccs has no guide hits, skipping read. >|> 20220329 04:25:46.730 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/56297281/ccs has no guide hits, skipping read. >|> 20220329 04:25:46.847 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/57213423/ccs has no guide hits, skipping read. >|> 20220329 04:25:47.221 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/62850768/ccs has no guide hits, skipping read. >|> 20220329 04:25:47.316 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/64621401/ccs has no guide hits, skipping read. >|> 20220329 04:25:47.316 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/64684535/ccs has no guide hits, skipping read. >|> 20220329 04:25:47.386 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/65798173/ccs has no guide hits, skipping read. >|> 20220329 04:25:47.477 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/66784045/ccs has no guide hits, skipping read. >|> 20220329 04:25:47.626 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/68160223/ccs has no guide hits, skipping read. >|> 20220329 04:25:47.923 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/73073689/ccs has no guide hits, skipping read. >|> 20220329 04:25:48.156 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/75497675/ccs has no guide hits, skipping read. >|> 20220329 04:25:48.184 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/75958150/ccs has no guide hits, skipping read. >|> 20220329 04:25:48.245 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/77203936/ccs has no guide hits, skipping read. >|> 20220329 04:25:48.277 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/77923753/ccs has no guide hits, skipping read. >|> 20220329 04:25:48.427 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/80217154/ccs has no guide hits, skipping read. >|> 20220329 04:25:48.679 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/82575535/ccs has no guide hits, skipping read. >|> 20220329 04:25:48.906 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/84609517/ccs has no guide hits, skipping read. >|> 20220329 04:25:49.105 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/87623363/ccs has no guide hits, skipping read. >|> 20220329 04:25:49.154 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/88410193/ccs has no guide hits, skipping read. >|> 20220329 04:25:49.483 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/91619958/ccs has no guide hits, skipping read. >|> 20220329 04:25:49.900 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/95225720/ccs has no guide hits, skipping read. >|> 20220329 04:25:49.950 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/96340783/ccs has no guide hits, skipping read. >|> 20220329 04:25:50.014 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/97255671/ccs has no guide hits, skipping read. >|> 20220329 04:25:50.186 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/99945178/ccs has no guide hits, skipping read. >|> 20220329 04:25:50.231 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/100925815/ccs has no guide hits, skipping read. >|> 20220329 04:25:50.235 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/100992556/ccs has no guide hits, skipping read. >|> 20220329 04:25:50.737 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/105974477/ccs has no guide hits, skipping read. >|> 20220329 04:25:51.441 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/115476633/ccs has no guide hits, skipping read. >|> 20220329 04:25:51.626 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/119146105/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.070 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/124321929/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.114 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/124978349/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.391 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/126878019/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.484 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/127992441/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.533 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/128649276/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.849 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/133432243/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.899 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/134350897/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.932 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/135071539/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.940 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/135332139/ccs has no guide hits, skipping read. >|> 20220329 04:25:53.132 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/137494935/ccs has no guide hits, skipping read. >|> 20220329 04:25:53.383 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/140641808/ccs has no guide hits, skipping read. >|> 20220329 04:25:53.743 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/141363825/ccs has no guide hits, skipping read. >|> 20220329 04:25:53.754 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/141625646/ccs has no guide hits, skipping read. >|> 20220329 04:25:54.080 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/146475194/ccs has no guide hits, skipping read. >|> 20220329 04:25:54.113 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/147131194/ccs has no guide hits, skipping read. >|> 20220329 04:25:54.230 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/148373698/ccs has no guide hits, skipping read. >|> 20220329 04:25:54.291 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/149162826/ccs has no guide hits, skipping read. >|> 20220329 04:25:54.322 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/150014348/ccs has no guide hits, skipping read. >|> 20220329 04:25:54.445 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/151063159/ccs has no guide hits, skipping read. >|> 20220329 04:25:54.460 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/151390380/ccs has no guide hits, skipping read. >|> 20220329 04:25:54.492 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/152175745/ccs has no guide hits, skipping read. >|> 20220329 04:25:54.681 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/154012268/ccs has no guide hits, skipping read. >|> 20220329 04:25:54.826 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/155976901/ccs has no guide hits, skipping read. >|> 20220329 04:25:54.830 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/156042642/ccs has no guide hits, skipping read. >|> 20220329 04:25:54.959 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/157746659/ccs has no guide hits, skipping read. >|> 20220329 04:25:54.971 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/158008603/ccs has no guide hits, skipping read. >|> 20220329 04:25:54.977 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/158140055/ccs has no guide hits, skipping read. >|> 20220329 04:25:55.156 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/160105275/ccs has no guide hits, skipping read. >|> 20220329 04:25:55.180 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/160629756/ccs has no guide hits, skipping read. >|> 20220329 04:25:55.592 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/164431373/ccs has no guide hits, skipping read. >|> 20220329 04:25:55.976 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/170920387/ccs has no guide hits, skipping read. >|> 20220329 04:25:56.008 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/171772258/ccs has no guide hits, skipping read. >|> 20220329 04:25:56.140 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/172231051/ccs has no guide hits, skipping read. >|> 20220329 04:25:56.592 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/174588241/ccs has no guide hits, skipping read. >|> 20220329 04:25:56.636 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/175507393/ccs has no guide hits, skipping read. >|> 20220329 04:25:56.637 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/175571543/ccs has no guide hits, skipping read. >|> 20220329 04:25:57.476 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/179504904/ccs has no guide hits, skipping read. >|> 20220329 04:25:57.487 -|- WARN -|- operator() -|- 0x7fb676472740|| -|- m64013e_210223_231140/179832753/ccs has no guide hits, skipping read. >|> 20220329 04:26:27.253 -|- WARN -|- BuildKFG -|- 0x7fb676472740|| -|- read: m64013e_210223_231140/107414737/ccs QV: 19.9859 skipped < Q20. >|> 20220329 04:26:28.175 -|- WARN -|- BuildKFG -|- 0x7fb676472740|| -|- read: m64013e_210223_231140/108464233/ccs QV: 19.9996 skipped < Q20. >|> 20220329 04:26:30.062 -|- WARN -|- BuildKFG -|- 0x7fb676472740|| -|- read: m64013e_210223_231140/110889197/ccs QV: 19.9951 skipped < Q20. >|> 20220329 04:26:35.724 -|- WARN -|- BuildKFG -|- 0x7fb676472740|| -|- read: m64013e_210223_231140/119014612/ccs QV: 19.9844 skipped < Q20. >|> 20220329 04:26:36.012 -|- WARN -|- BuildKFG -|- 0x7fb676472740|| -|- read: m64013e_210223_231140/119472560/ccs QV: 19.9796 skipped < Q20. >|> 20220329 04:26:51.159 -|- WARN -|- BuildKFG -|- 0x7fb676472740|| -|- read: m64013e_210223_231140/139788968/ccs QV: 19.9918 skipped < Q20. >|> 20220329 04:26:53.391 -|- WARN -|- BuildKFG -|- 0x7fb676472740|| -|- read: m64013e_210223_231140/14289151/ccs QV: 19.9934 skipped < Q20. >|> 20220329 04:27:01.100 -|- WARN -|- BuildKFG -|- 0x7fb676472740|| -|- read: m64013e_210223_231140/153356554/ccs QV: 19.9983 skipped < Q20. >|> 20220329 04:27:12.772 -|- WARN -|- BuildKFG -|- 0x7fb676472740|| -|- read: m64013e_210223_231140/169806217/ccs QV: 19.9862 skipped < Q20. >|> 20220329 04:27:40.329 -|- WARN -|- BuildKFG -|- 0x7fb676472740|| -|- read: m64013e_210223_231140/43386569/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:27:44.933 -|- WARN -|- BuildKFG -|- 0x7fb676472740|| -|- read: m64013e_210223_231140/4916672/ccs QV: 19.9831 skipped < Q20. >|> 20220329 04:28:10.843 -|- WARN -|- BuildKFG -|- 0x7fb676472740|| -|- read: m64013e_210223_231140/82772767/ccs QV: 19.9931 skipped < Q20. >|> 20220329 04:28:19.108 -|- WARN -|- BuildKFG -|- 0x7fb676472740|| -|- read: m64013e_210223_231140/94306401/ccs QV: 19.9907 skipped < Q20. >|> 20220329 04:28:20.094 -|- WARN -|- BuildKFG -|- 0x7fb676472740|| -|- read: m64013e_210223_231140/95750272/ccs QV: 19.9984 skipped < Q20. >|> 20220329 04:30:54.993 -|- INFO -|- RunAlignments -|- 0x7fb676472740|| -|- Finished 550814 valid pairwise alignments in 144617 ms >|> 20220329 04:34:04.555 -|- INFO -|- ChimeraDetection -|- 0x7fb676472740|| -|- Running UCHIME algorithm. >|> 20220329 04:34:04.640 -|- INFO -|- LaaRunner -|- 0x7fb676472740|| -|- Finished without error. >|> 20220329 04:34:04.640 -|- INFO -|- LaaRunner -|- 0x7fb676472740|| -|- Real time : 8m 21s >|> 20220329 04:34:04.640 -|- INFO -|- LaaRunner -|- 0x7fb676472740|| -|- CPU time : 47m 13s >|> 20220329 04:34:04.640 -|- INFO -|- LaaRunner -|- 0x7fb676472740|| -|- Peak RSS : 27.267 GB