>|> 20220329 04:25:45.567 -|- INFO -|- LaaRunner -|- 0x7fe99270f740|| -|- Welcome to pbaa. >|> 20220329 04:25:45.572 -|- INFO -|- GraphProcessing -|- 0x7fe99270f740|| -|- 1 sequence entries. >|> 20220329 04:25:45.572 -|- INFO -|- GraphProcessing -|- 0x7fe99270f740|| -|- 4507 nodes before filtering. >|> 20220329 04:25:45.572 -|- INFO -|- GraphProcessing -|- 0x7fe99270f740|| -|- 4507 nodes after filtering. >|> 20220329 04:25:45.572 -|- INFO -|- GetGuideInfo -|- 0x7fe99270f740|| -|- Processing 1 guide loci. >|> 20220329 04:25:45.573 -|- INFO -|- GetGuideInfo -|- 0x7fe99270f740|| -|- There are 4507 unique kmers within guide sequence(s). >|> 20220329 04:25:45.851 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/2425658/ccs has no guide hits, skipping read. >|> 20220329 04:25:46.320 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/9373138/ccs has no guide hits, skipping read. >|> 20220329 04:25:46.500 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/12910991/ccs has no guide hits, skipping read. >|> 20220329 04:25:46.740 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/18285567/ccs has no guide hits, skipping read. >|> 20220329 04:25:46.871 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/19924463/ccs has no guide hits, skipping read. >|> 20220329 04:25:46.987 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/22021723/ccs has no guide hits, skipping read. >|> 20220329 04:25:47.087 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/24315605/ccs has no guide hits, skipping read. >|> 20220329 04:25:47.220 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/27329751/ccs has no guide hits, skipping read. >|> 20220329 04:25:47.382 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/29361735/ccs has no guide hits, skipping read. >|> 20220329 04:25:47.924 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/36700216/ccs has no guide hits, skipping read. >|> 20220329 04:25:47.959 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/37356920/ccs has no guide hits, skipping read. >|> 20220329 04:25:48.293 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/42928166/ccs has no guide hits, skipping read. >|> 20220329 04:25:48.926 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/48826610/ccs has no guide hits, skipping read. >|> 20220329 04:25:49.817 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/57739926/ccs has no guide hits, skipping read. >|> 20220329 04:25:49.936 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/59179763/ccs has no guide hits, skipping read. >|> 20220329 04:25:50.383 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/64620421/ccs has no guide hits, skipping read. >|> 20220329 04:25:50.477 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/65734187/ccs has no guide hits, skipping read. >|> 20220329 04:25:51.467 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/73205465/ccs has no guide hits, skipping read. >|> 20220329 04:25:51.905 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/75236239/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.034 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/78383769/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.216 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/81070872/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.217 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/81136046/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.385 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/83362128/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.407 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/84085301/ccs has no guide hits, skipping read. >|> 20220329 04:25:52.783 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/90441059/ccs has no guide hits, skipping read. >|> 20220329 04:25:53.003 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/92604896/ccs has no guide hits, skipping read. >|> 20220329 04:25:54.444 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/108725842/ccs has no guide hits, skipping read. >|> 20220329 04:25:54.499 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/109773474/ccs has no guide hits, skipping read. >|> 20220329 04:25:55.262 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/117834769/ccs has no guide hits, skipping read. >|> 20220329 04:25:56.742 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/132712870/ccs has no guide hits, skipping read. >|> 20220329 04:25:59.064 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/151259439/ccs has no guide hits, skipping read. >|> 20220329 04:25:59.255 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/156042197/ccs has no guide hits, skipping read. >|> 20220329 04:25:59.494 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/160041645/ccs has no guide hits, skipping read. >|> 20220329 04:25:59.572 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/161941677/ccs has no guide hits, skipping read. >|> 20220329 04:25:59.643 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/162924490/ccs has no guide hits, skipping read. >|> 20220329 04:25:59.823 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/165413324/ccs has no guide hits, skipping read. >|> 20220329 04:25:59.910 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/166987332/ccs has no guide hits, skipping read. >|> 20220329 04:25:59.994 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/167773800/ccs has no guide hits, skipping read. >|> 20220329 04:26:00.077 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/168821562/ccs has no guide hits, skipping read. >|> 20220329 04:26:00.148 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/170133766/ccs has no guide hits, skipping read. >|> 20220329 04:26:00.268 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/171706523/ccs has no guide hits, skipping read. >|> 20220329 04:26:00.383 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/173869250/ccs has no guide hits, skipping read. >|> 20220329 04:26:00.611 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/177407240/ccs has no guide hits, skipping read. >|> 20220329 04:26:00.613 -|- WARN -|- operator() -|- 0x7fe99270f740|| -|- m64013e_210223_231140/177472498/ccs has no guide hits, skipping read. >|> 20220329 04:26:19.089 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/103023972/ccs rounded length: 17500 skipped > 100 bp. >|> 20220329 04:26:19.846 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/104596130/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:26:22.732 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/110166550/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:26:23.800 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/112001669/ccs QV: 19.9852 skipped < Q20. >|> 20220329 04:26:25.111 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/11469279/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:26:26.899 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/1180855/ccs QV: 19.9963 skipped < Q20. >|> 20220329 04:26:28.767 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/121636722/ccs QV: 19.9837 skipped < Q20. >|> 20220329 04:26:29.971 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/124126655/ccs QV: 19.9884 skipped < Q20. >|> 20220329 04:26:30.697 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/125371491/ccs QV: 19.9876 skipped < Q20. >|> 20220329 04:26:30.977 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/125700292/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:26:31.057 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/12584345/ccs rounded length: 17500 skipped > 100 bp. >|> 20220329 04:26:31.775 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/127205994/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:26:33.453 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/130286856/ccs QV: 19.9988 skipped < Q20. >|> 20220329 04:26:33.646 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/130614314/ccs QV: 19.999 skipped < Q20. >|> 20220329 04:26:37.749 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/137430382/ccs QV: 19.9913 skipped < Q20. >|> 20220329 04:26:41.450 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/14419405/ccs QV: 19.9947 skipped < Q20. >|> 20220329 04:26:41.623 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/144443440/ccs QV: 19.9926 skipped < Q20. >|> 20220329 04:26:42.805 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/14681586/ccs rounded length: 17500 skipped > 100 bp. >|> 20220329 04:26:43.371 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/147917005/ccs QV: 19.9869 skipped < Q20. >|> 20220329 04:26:45.142 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/151128469/ccs QV: 19.9967 skipped < Q20. >|> 20220329 04:26:48.339 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/157352359/ccs QV: 19.9798 skipped < Q20. >|> 20220329 04:26:49.661 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/15991749/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:26:55.523 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/171312423/ccs QV: 19.9904 skipped < Q20. >|> 20220329 04:26:56.095 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/172361076/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:26:56.844 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/173867720/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:26:57.078 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/174392588/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:26:57.106 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/174458547/ccs QV: 19.9972 skipped < Q20. >|> 20220329 04:26:59.386 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/178914451/ccs QV: 19.9979 skipped < Q20. >|> 20220329 04:27:01.498 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/19924607/ccs QV: 19.9815 skipped < Q20. >|> 20220329 04:27:01.628 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/20251583/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:27:06.343 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/28967197/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:27:07.925 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/31918378/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:27:08.091 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/32180162/ccs QV: 19.9974 skipped < Q20. >|> 20220329 04:27:08.632 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/33228357/ccs QV: 19.9982 skipped < Q20. >|> 20220329 04:27:09.367 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/34472204/ccs QV: 19.9901 skipped < Q20. >|> 20220329 04:27:10.607 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/3671910/ccs QV: 19.9892 skipped < Q20. >|> 20220329 04:27:10.985 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/37553490/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:27:14.075 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/43123786/ccs QV: 19.9968 skipped < Q20. >|> 20220329 04:27:14.267 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/43385066/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:27:20.085 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/53936367/ccs QV: 19.9923 skipped < Q20. >|> 20220329 04:27:20.621 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/54790894/ccs QV: 19.9958 skipped < Q20. >|> 20220329 04:27:22.497 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/58197143/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:27:22.770 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/58656392/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:27:26.120 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/64424125/ccs QV: 19.9901 skipped < Q20. >|> 20220329 04:27:28.769 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/68356134/ccs QV: 19.9978 skipped < Q20. >|> 20220329 04:27:29.333 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/6948012/ccs QV: 19.9914 skipped < Q20. >|> 20220329 04:27:33.641 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/77203438/ccs QV: 19.9914 skipped < Q20. >|> 20220329 04:27:36.764 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/82903407/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:27:42.461 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/93194214/ccs rounded length: 17500 skipped > 100 bp. >|> 20220329 04:27:44.486 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/97191166/ccs QV: 19.9952 skipped < Q20. >|> 20220329 04:27:44.772 -|- WARN -|- BuildKFG -|- 0x7fe99270f740|| -|- read: m64013e_210223_231140/97716553/ccs rounded length: 17400 skipped > 100 bp. >|> 20220329 04:29:14.889 -|- INFO -|- RunAlignments -|- 0x7fe99270f740|| -|- Finished 998724 valid pairwise alignments in 83205 ms >|> 20220329 04:31:03.794 -|- INFO -|- ChimeraDetection -|- 0x7fe99270f740|| -|- Running UCHIME algorithm. >|> 20220329 04:31:04.461 -|- INFO -|- LaaRunner -|- 0x7fe99270f740|| -|- Finished without error. >|> 20220329 04:31:04.461 -|- INFO -|- LaaRunner -|- 0x7fe99270f740|| -|- Real time : 5m 18s >|> 20220329 04:31:04.461 -|- INFO -|- LaaRunner -|- 0x7fe99270f740|| -|- CPU time : 30m 39s >|> 20220329 04:31:04.461 -|- INFO -|- LaaRunner -|- 0x7fe99270f740|| -|- Peak RSS : 24.763 GB