SAMPLE
    Universal Human Reference RNA (Agilent) + SIRV Isoform Mix E0 (Lexogen)

METHODS
    Library prep     Iso-Seq Template Preparation for Sequel Systems (PN 101-070-200)
    Sequencing       Sequel System II with "Early Access" binding kit (101-490-800) and chemistry (101-490-900)
    Analysis         SMRTlink 7.0 "IsoSeq With Mapping protocol" with hg38+SIRV combined reference genome

FILES


    Users are encouraged to work with the mapped, unique transcripts in GFF and FASTQ format provided in the "PolishedMappedTranscripts" subfolder. The two raw movie files are in "subreads" subfolder. The intermediate data, full-length reads, are available in "FullLengthReads" folder.


    PolishedMappedTranscripts/
             out.abundance.txt
             out.fastq
             out.gff


    FullLengthReads/
             flnc.bam

    subreads/
             m64012_181221_231243.subreads.bam
             m64012_181221_231243.subreads.bam.pbi
             m64012_181222_192540.subreads.bam
             m64012_181222_192540.subreads.bam.pbi