OVERVIEW This dataset is an example of the output format for the Sequel® IIe System. METHODS Samples Four gut microbiome pooled standards (see movie information) Library prep SMRTbell® Express Template Prep Kit 2.0 + Enzyme Clean Up Kit 2.0 Sequencing Sequel IIe System with Sequel II Binding Kit 2.2 chemistry 2.0 Run time 30hr movie; 2 hr pre-extension Software ICS v10.0, SMRT® Link v10.1 and CCS v6.0.0 MOVIE m64011_210224_000525 - The BioCollective pooled gut microbiome sample 138274; 10.3kb insert m64011_210225_094432 - The BioCollective pooled gut microbiome sample 138390; 9.2kb insert m64011_210226_210143 - The BioCollective pooled gut microbiome sample 139369; 9.8kb insert m64011_210228_064650 - The BioCollective pooled gut microbiome sample 139445; 8.6kb insert DATA FILES [movie].hifi_reads.bam HiFi reads, subset of [movie].reads.bam [movie].hifi_reads.fastq.gz HiFi reads, FASTQ format [movie].hifi_reads.fasta.gz HiFi reads, FASTA format