Index of /public/dataset/Sequel-IIe-202104/metagenomics
Name Last modified Size Description
Parent Directory -
README.txt 2021-04-22 14:37 1.3K
m64011_210224_000525.hifi_reads.bam 2021-03-05 16:54 11G
m64011_210224_000525.hifi_reads.fasta.tar.gz 2021-04-22 16:12 5.4G
m64011_210224_000525.hifi_reads.fastq.tar.gz 2021-04-22 16:24 11G
m64011_210225_094432.hifi_reads.bam 2021-03-05 16:58 9.4G
m64011_210225_094432.hifi_reads.fasta.tar.gz 2021-04-22 17:10 4.5G
m64011_210225_094432.hifi_reads.fastq.tar.gz 2021-04-22 17:31 9.2G
m64011_210226_210143.hifi_reads.bam 2021-03-05 17:03 12G
m64011_210226_210143.hifi_reads.fasta.tar.gz 2021-04-22 18:19 5.5G
m64011_210226_210143.hifi_reads.fastq.tar.gz 2021-04-22 18:53 12G
m64011_210228_064650.hifi_reads.bam 2021-03-05 17:07 9.4G
m64011_210228_064650.hifi_reads.fasta.tar.gz 2021-04-23 10:22 4.4G
m64011_210228_064650.hifi_reads.fastq.tar.gz 2021-04-23 10:33 9.2G
OVERVIEW
This dataset is an example of the output format for the Sequel® IIe System.
METHODS
Samples Four gut microbiome pooled standards (see movie information)
Library prep SMRTbell® Express Template Prep Kit 2.0 + Enzyme Clean Up Kit 2.0
Sequencing Sequel IIe System with Sequel II Binding Kit 2.2 chemistry 2.0
Run time 30hr movie; 2 hr pre-extension
Software ICS v10.0, SMRT® Link v10.1 and CCS v6.0.0
MOVIE
m64011_210224_000525 - The BioCollective pooled gut microbiome sample 138274; 10.3kb insert
m64011_210225_094432 - The BioCollective pooled gut microbiome sample 138390; 9.2kb insert
m64011_210226_210143 - The BioCollective pooled gut microbiome sample 139369; 9.8kb insert
m64011_210228_064650 - The BioCollective pooled gut microbiome sample 139445; 8.6kb insert
DATA FILES
[movie].hifi_reads.bam HiFi reads, subset of [movie].reads.bam
[movie].hifi_reads.fastq.gz HiFi reads, FASTQ format
[movie].hifi_reads.fasta.gz HiFi reads, FASTA format