OVERVIEW This dataset is an example of the output format for the Sequel® IIe System. METHODS Sample HG002 extracted DNA Library prep SMRTbell® Express 2.0 + Enzyme Clean Up Sequencing Sequel IIe System with chemistry 2.0 Run time 30 hrs movie with 2 hr pre-extension Software ICS v10.0, SMRT® Link v10.0 and CCS v5.0.0 MOVIES m64012_201115_115917 - no kinetic information, default m64012_201204_044926 - kinetic tags enabled (fi, fp, fn, ri, rp, rn) DATA FILES [movie].reads.bam Sequel IIe System output with one read per productive ZMW containing: 1. HiFi Reads (QV 20 or higher) 2. Lower-quality but still polished consensus reads (QV 1 - QV 20) 3. Unpolished consensus reads (RQ=-1) 4. Single-pass subreads unaltered (RQ=-1) [movie].hifi_reads.bam HiFi reads, subset of [movie].reads.bam [movie].hifi_reads.fastq.gz HiFi reads, FASTQ format [movie].hifi_reads.fasta.gz HiFi reads, FASTA format The [movie].reads.bam contains HiFi Reads and non-HiFi reads. For tools outside SMRT Link that except only HiFi reads, use [movie].hifi_reads.bam/fastq/fasta.