Index of /public/dataset/RC0_1cell_2017
Name Last modified Size Description
Parent Directory -
m54086_170204_081430.subreadset.xml 2017-02-04 11:37 10K
m54086_170204_081430.subreads.bam.pbi 2017-02-04 11:39 23M
m54086_170204_081430.subreads.bam 2017-02-04 11:39 8.8G
m54086_170204_081430.sts.xml 2017-02-04 11:41 96K
m54086_170204_081430.scraps.bam.pbi 2017-02-04 11:41 35M
m54086_170204_081430.scraps.bam 2017-02-04 11:41 12G
m54086_170204_081430.adapters.fasta 2017-02-04 11:42 58
isoseq_nfl.fasta 2017-06-17 22:53 248M
isoseq_flnc.fasta 2017-06-17 22:53 496M
README.txt 2017-08-08 13:47 2.0K
README (Last Updated 08/08/2017)
This README file describes the contents in this directory.
This dataset contains raw and intermediate data for advanced users to test ToFU2.
2. LIBRARY PREPARATION AND SEQUENCING
The data contains a single cell of Lexogen RC0 (https://www.lexogen.com/sirvs/)
which is a mixture of spiked in SIRV and ERCC into UHRR. Please visit the Lexogen
SIRV website for more details.
The cDNA library was made from total RNA using the Clontech SMARTer cDNA
synthesis kit followed by large-scale PCR using the Takara PrimeStar GXL polymerase.
The RC0 non-size-selected SMRTBell library was sequenced with chemistry 2.0 using
Magbead loading (50pM on plate) for a 6hr movie.
For a full procedure please see:
3. FILE DESCRIPTION
All files starting with m54086_170204_081430.* are the raw data from the 1 cell of RC0.
isoseq_flnc.fasta --- full-length reads after running Iso-Seq classify.
isoseq_nfl.fasta --- non-full length reads after running Iso-Seq classify.
To run ToFU2, please follow the tutorial at:
4. Alternative Download Location
The same data is also stored in Zenodo: https://doi.org/10.5281/zenodo.832942
For Research Use Only. Not for use in diagnostic procedures. Copyright 2017, Pacific Biosciences of California, Inc. All rights reserved. The data provided in these files is subject to change without notice and Pacific Biosciences assumes no responsibility for any errors or omissions. Certain notices, terms, conditions and/or use restrictions may pertain to your use of Pacific Biosciences data, products and/or third party products. Please refer to the applicable Pacific Biosciences Terms and Conditions of Sale and to the applicable license terms at http://www.pacificbiosciences.com/licenses.html.