Index of /public/dataset/HG002/Sequel-II-201910/HG002-HiFi-VariantDetection
Name Last modified Size Description
Parent Directory -
variants/ 2019-10-01 23:29 -
alignments/ 2019-10-01 23:28 -
hifireads/ 2019-10-01 15:13 -
MD5.txt 2019-10-02 00:09 680
README.txt 2019-10-01 23:52 608
SAMPLE
HG002 extracted DNA
METHODS
Shearing 20 kb with Megaruptor
Library prep TPK 1.0
Size selection SageELF Fraction 4 (target: 15 kb)
Sequencing Sequel II System with 2.0 chemistry
Run time 30 hrs per SMRT Cell
Reference hs37d5 (GRCh37 with decoy)
CCS "Circular Consensus Sequencing" analysis in SMRT Link v8.0 (ccs 4.0.0)
SV Calling "Structural Variant Calling" analysis in SMRT Link v8.0
Variant Calling DeepVariant 0.8.0
Variant Phasing WhatsHap 0.18
DOWNLOAD
MD5 checksums are listed in MD5.txt