Index of /public/dataset/HG002/Sequel-II-201910/HG002+HG005-Multiplex-SvDetection
Name Last modified Size Description
Parent Directory -
alignments/ 2019-11-08 14:17 -
subreads/ 2019-11-08 11:22 -
variants/ 2019-11-08 15:48 -
README.txt 2019-11-08 11:47 598
MD5.txt 2019-11-08 15:51 724
SAMPLE
HG002 extracted DNA
HG005 extracted DNA
METHODS
Shearing 30 kb with Megaruptor
Library prep SMRTbell Express 2.0 with barcoded adapters (bc1015 for HG002, bc1019 for HG005)
Size selection >15 kb with BluePippin
Sequencing Sequel II System with 2.0 chemistry
Run time 15 hrs per SMRT Cell
Reference hs37d5 (GRCh37 with decoy)
Demultiplexing "Demultiplex Barcodes" analysis in SMRT Link v8.0
SV Calling "Structural Variant Calling" analysis in SMRT Link v8.0
DOWNLOAD
MD5 checksums are listed in MD5.txt