Index of /public/dataset/HG002/Sequel-II-201910/HG002+HG005-Multiplex-SvDetection

Icon  Name                                    Last modified      Size  Description
[PARENTDIR] Parent Directory - [DIR] subreads/ 2019-11-08 11:22 - [DIR] alignments/ 2019-11-08 14:17 - [DIR] variants/ 2019-11-08 15:48 - [TXT] README.txt 2019-11-08 11:47 598 [TXT] MD5.txt 2019-11-08 15:51 724
SAMPLE
    HG002 extracted DNA
    HG005 extracted DNA

METHODS
    Shearing         30 kb with Megaruptor
    Library prep     SMRTbell Express 2.0 with barcoded adapters (bc1015 for HG002, bc1019 for HG005)
    Size selection   >15 kb with BluePippin
    Sequencing       Sequel II System with 2.0 chemistry
    Run time         15 hrs per SMRT Cell
    Reference        hs37d5 (GRCh37 with decoy)
    Demultiplexing   "Demultiplex Barcodes" analysis in SMRT Link v8.0
    SV Calling       "Structural Variant Calling" analysis in SMRT Link v8.0

DOWNLOAD
    MD5 checksums are listed in MD5.txt