LOCUS       6520_pRep2-Cap9         7324 bp    DNA     circular SYN 02-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7324)
  AUTHORS   Marc Hemberger
  TITLE     Direct Submission
  JOURNAL   Exported Apr 2, 2025 from SnapGene 8.0.3
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..7324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             146..2011
                     /codon_start=1
                     /label=Rep78
                     /translation="MPGFYEIVIKVPSDLDEHLPGISDSFVNWVAEKEWELPPDSDMDL
                     NLIEQAPLTVAEKLQRDFLTEWRRVSKAPEALFFVQFEKGESYFHMHVLVETTGVKSMV
                     LGRFLSQIREKLIQRIYRGIEPTLPNWFAVTKTRNGAGGGNKVVDECYIPNYLLPKTQP
                     ELQWAWTNMEQYLSACLNLTERKRLVAQHLTHVSQTQEQNKENQNPNSDAPVIRSKTSA
                     RYMELVGWLVDKGITSEKQWIQEDQASYISFNAASNSRSQIKAALDNAGKIMSLTKTAP
                     DYLVGQQPVEDISSNRIYKILELNGYDPQYAASVFLGWATKKFGKRNTIWLFGPATTGK
                     TNIAEAIAHTVPFYGCVNWTNENFPFNDCVDKMVIWWEEGKMTAKVVESAKAILGGSKV
                     RVDQKCKSSAQIDPTPVIVTSNTNMCAVIDGNSTTFEHQQPLQDRMFKFELTRRLDHDF
                     GKVTKQEVKDFFRWAKDHVVEVEHEFYVKKGGAKKRPAPSDADISEPKRVRESVAQPST
                     SDAEASINYADRYQNKCSRHVGMNLMLFPCRQCERMNQNSNICFTHGQKDCLECFPVSE
                     SQPVSVVKKAYQKLCYIHHIMGKVPDACTACDLVNVDLDDCIFEQ"
     primer_bind     complement(181..200)
                     /label=9002_Rep78_falsch_F
     primer_bind     complement(226..243)
                     /label=18535_AAV2_Rep_Seq_R
     primer_bind     322..339
                     /label=14129_Rep_Seq_For
     primer_bind     805..824
                     /label=13265_AAV2-Rep78_Seq_For
     primer_bind     808..826
                     /label=11822_11842_GLucII_Seq_For
     primer_bind     complement(884..904)
                     /label=11843_GLucII_Seq_Rev
     primer_bind     complement(942..959)
                     /label=16153_8111_Rep_Seq_Rev
     primer_bind     946..965
                     /label=6658_Rep78_Seq-R
     primer_bind     1633..1653
                     /label=10778_Rep_Seq_F1
     primer_bind     1747..1764
                     /label=14216_Rep2_Seq_For
     primer_bind     1935..1952
                     /label=12968_Rep78end_Seq_For
     primer_bind     complement(1935..1952)
                     /label=15580_Rep78_3'_Seq_Rev
     primer_bind     1963..1982
                     /label=9003_Rep78_right_F
     primer_bind     1963..1982
                     /label=9003_Rep78_right_F (1)
     primer_bind     1969..1988
                     /label=11145_RC3_F1
     CDS             2028..4238
                     /codon_start=1
                     /label=Cap9 WT
                     /label=cap9
                     /note="/vntifkey=21"
                     /translation="MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGL
                     VLPGYKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADAEFQERL
                     KEDTSFGGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSG
                     AQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVG
                     SSSGNWHCDSQWLGDRVITTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPW
                     GYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTS
                     TVQVFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEY
                     FPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLIDQYLYYLSKTINGSGQNQ
                     QTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVSTTVTQNNNSEFAWPGASSWALNGRNSL
                     MNPGPAMASHKEGEDRFFPLSGSLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATES
                     YGQVATNHQSAQAQAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFHPSPLM
                     GGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVSVEIEWELQKENSKR
                     WNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRPIGTRYLTRNL"
     primer_bind     complement(2312..2321)
                     /label=15581_Cap5_Seq_Rev
     primer_bind     2365..2386
                     /label=9729_Seq_Cap_AAV1_F1
     primer_bind     2365..2386
                     /label=9729_Seq_Cap_AAV1_F1 (1)
     primer_bind     2435..2454
                     /label=10779_RC1_Seq_F2
     primer_bind     2437..2456
                     /label=10785_RC4_Seq_F2
     primer_bind     2479..2502
                     /label=9733_Seq_Cap_AAV9_F1
     primer_bind     2479..2502
                     /label=9733_Seq_Cap_AAV9_F1 (1)
     primer_bind     complement(2663..2683)
                     /label=9732_Seq_Cap_AAV9_R
     primer_bind     2700..2718
                     /label=13005_Cap5_Seq_For
     primer_bind     2738..2756
                     /label=13270#
     primer_bind     complement(2769..2786)
                     /label=13010_Cap8_Seq_Rev
     primer_bind     complement(2888..2907)
                     /label=14219_Cap3_Seq_Rev
     primer_bind     3088..3109
                     /label=9734_Seq_Cap_AAV9_F2
     primer_bind     3088..3109
                     /label=9734_Seq_Cap_AAV9_F2 (1)
     primer_bind     3679..3702
                     /label=9735_Seq_Cap_AAV9_F3
     primer_bind     3679..3702
                     /label=9735_Seq_Cap_AAV9_F3 (1)
     primer_bind     4084..4103
                     /label=10784_RC2_Seq_F4
     primer_bind     4084..4103
                     /label=10793_RC8_Seq_F4
     primer_bind     complement(4286..4302)
                     /label=12649_Cap_Seq_Rev
     primer_bind     4321..4342
                     /label=16154_8111_Seq_For
     primer_bind     complement(4322..4342)
                     /label=13267_Cap-3'UTR_Seq_Rev
     primer_bind     complement(4325..4345)
                     /label=11777_Seq_Primer_Rev
     primer_bind     complement(4468..4486)
                     /label=11776_Seq_Primer_Rev
     primer_bind     complement(4575..4592)
                     /label=1704_M13_rev
     primer_bind     complement(4660..4679)
                     /label=17993_JJ023
     primer_bind     complement(4724..4741)
                     /label=10464_pENTR_Seq_R
     primer_bind     complement(4894..4912)
                     /label=7521_attL_Site-Seq_Rev
     primer_bind     complement(4941..4959)
                     /label=13923_GSPT1_Seq_Rev (pMA-backbone)
     primer_bind     4963..4983
                     /label=7606_pENTR_223.1_R1
     primer_bind     complement(4969..4988)
                     /label=6639_ColEI_Rev
     primer_bind     complement(4969..4988)
                     /label=6639_ColEI_Rev (1)
     rep_origin      complement(4978..5566)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication; Facilitates plasmid replication in E. coli; 
                     regulates low-copy plasmid number when Rop protein is 
                     presence (15-20) and median-copy plasmid number when Rop is
                     absent (100-300)."
     primer_bind     complement(5016..5035)
                     /label=6541_Amp_Rev_P2
     primer_bind     complement(5025..5044)
                     /label=4294_pUC_ori_Seq_R
     primer_bind     complement(5025..5044)
                     /label=10300_3746_L-ITR_F
     primer_bind     complement(5038..5056)
                     /label=14411_ori_Seq_FOR
     primer_bind     complement(5051..5072)
                     /label=11085_ITR_Seq_1
     primer_bind     5159..5178
                     /label=14154_ori_qPCR_R
     primer_bind     complement(5288..5307)
                     /label=14153_ori_qPCR_F
     primer_bind     complement(5492..5511)
                     /label=4016_Col-E1_Origin_F
     primer_bind     5492..5511
                     /label=18302_Kana_Seq_for
     primer_bind     5510..5524
                     /label=4016_Col-E1_Origin_F
     primer_bind     complement(5611..5642)
                     /label=6491_ColE1_Seq_R
     primer_bind     complement(5617..5641)
                     /label=18633_Seq_Rep2Cap2_rev
     primer_bind     5635..5672
                     /label=16905_HiFi.REV
     CDS             complement(join(5737..6528,6529..6597))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5940..5959
                     /label=7632_Amp_qPCR_R
     primer_bind     complement(6073..6092)
                     /label=7631_Amp_qPCR_F
     primer_bind     6084..6102
                     /label=6542_Amp_Mitte_Seq_F
     primer_bind     complement(6181..6202)
                     /label=2473_BLAs_F2_Seq
     primer_bind     complement(6301..6325)
                     /label=15158_AmpR_Seq_F
     primer_bind     6361..6381
                     /label=6520_Amp_F_3309
     primer_bind     complement(6400..6429)
                     /label=6492_Amp_Seq_ R
     promoter        complement(6598..6702)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6633..6654
                     /label=14825_AmpProm_Seq_Rev
     primer_bind     6634..6654
                     /label=14477_Kana_Seq_R
     rep_origin      complement(6728..7183)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6792..6811)
                     /label=14155_M13 ori_qPCR_F
     primer_bind     6898..6917
                     /label=3423_F1-Ori_R
     primer_bind     complement(6898..6917)
                     /label=15222_F1_ori_F
     primer_bind     6914..6933
                     /label=14233_M13ori_qPCR_R2
     primer_bind     complement(7033..7052)
                     /label=14232_M13ori_qPCR_F2
     primer_bind     complement(7034..7054)
                     /label=14769_BAd19a_Seq_R
     primer_bind     7059..7079
                     /label=3129_F1_ori_F
     primer_bind     7116..7132
                     /label=11087_ITR_Seq_3
     primer_bind     7133..7149
                     /label=14410_F1ori_Seq_Rev
     primer_bind     7231..7250
                     /label=17994_JJ022
     primer_bind     complement(7244..7263)
                     /label=18303_Kana_Seq_rev
     primer_bind     7245..7264
                     /label=12117_P5078/79_seq_LTR_F
     primer_bind     7246..7276
                     /label=6489_RSV_Seq_F
     primer_bind     7294..7318
                     /label=9961_pENTR_Geneart_Seq_F
     primer_bind     join(7324,1..17)
                     /label=7071_M13-fwd
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtaccg
       61 ggccccccct cgatcgaggt cgacggtatc gggggagctc gcagggtctc cattttgaag
      121 cgggaggttt gaacgcgcag ccgccatgcc ggggttttac gagattgtga ttaaggtccc
      181 cagcgacctt gacgagcatc tgcccggcat ttctgacagc tttgtgaact gggtggccga
      241 gaaggaatgg gagttgccgc cagattctga catggatctg aatctgattg agcaggcacc
      301 cctgaccgtg gccgagaagc tgcagcgcga ctttctgacg gaatggcgcc gtgtgagtaa
      361 ggccccggag gctcttttct ttgtgcaatt tgagaaggga gagagctact tccacatgca
      421 cgtgctcgtg gaaaccaccg gggtgaaatc catggttttg ggacgtttcc tgagtcagat
      481 tcgcgaaaaa ctgattcaga gaatttaccg cgggatcgag ccgactttgc caaactggtt
      541 cgcggtcaca aagaccagaa atggcgccgg aggcgggaac aaggtggtgg atgagtgcta
      601 catccccaat tacttgctcc ccaaaaccca gcctgagctc cagtgggcgt ggactaatat
      661 ggaacagtat ttaagcgcct gtttgaatct cacggagcgt aaacggttgg tggcgcagca
      721 tctgacgcac gtgtcgcaga cgcaggagca gaacaaagag aatcagaatc ccaattctga
      781 tgcgccggtg atcagatcaa aaacttcagc caggtacatg gagctggtcg ggtggctcgt
      841 ggacaagggg attacctcgg agaagcagtg gatccaggag gaccaggcct catacatctc
      901 cttcaatgcg gcctccaact cgcggtccca aatcaaggct gccttggaca atgcgggaaa
      961 gattatgagc ctgactaaaa ccgcccccga ctacctggtg ggccagcagc ccgtggagga
     1021 catttccagc aatcggattt ataaaatttt ggaactaaac gggtacgatc cccaatatgc
     1081 ggcttccgtc tttctgggat gggccacgaa aaagttcggc aagaggaaca ccatctggct
     1141 gtttgggcct gcaactaccg ggaagaccaa catcgcggag gccatagccc acactgtgcc
     1201 cttctacggg tgcgtaaact ggaccaatga gaactttccc ttcaacgact gtgtcgacaa
     1261 gatggtgatc tggtgggagg aggggaagat gaccgccaag gtcgtggagt cggccaaagc
     1321 cattctcgga ggaagcaagg tgcgcgtgga ccagaaatgc aagtcctcgg cccagataga
     1381 cccgactccc gtgatcgtca cctccaacac caacatgtgc gccgtgattg acgggaactc
     1441 aacgaccttc gaacaccagc agccgttgca agaccggatg ttcaaatttg aactcacccg
     1501 ccgtctggat catgactttg ggaaggtcac caagcaggaa gtcaaagact ttttccggtg
     1561 ggcaaaggat cacgtggttg aggtggagca tgaattctac gtcaaaaagg gtggagccaa
     1621 gaaaagaccc gcccccagtg acgcagatat aagtgagccc aaacgggtgc gcgagtcagt
     1681 tgcgcagcca tcgacgtcag acgcggaagc ttcgatcaac tacgcagaca ggtaccaaaa
     1741 caaatgttct cgtcacgtgg gcatgaatct gatgctgttt ccctgcagac aatgcgagag
     1801 aatgaatcag aattcaaata tctgcttcac tcacggacag aaagactgtt tagagtgctt
     1861 tcccgtgtca gaatctcaac ccgtttctgt cgtcaaaaag gcgtatcaga aactgtgcta
     1921 cattcatcat atcatgggaa aggtgccaga cgcttgcact gcctgcgatc tggtcaatgt
     1981 ggatttggat gactgcatct ttgaacaata aatgatttaa atcaggtatg gctgccgatg
     2041 gttatcttcc agattggctc gaggacaacc ttagtgaagg aattcgcgag tggtgggctt
     2101 tgaaacctgg agcccctcaa cccaaggcaa atcaacaaca tcaagacaac gctcgaggtc
     2161 ttgtgcttcc gggttacaaa taccttggac ccggcaacgg actcgacaag ggggagccgg
     2221 tcaacgcagc agacgcggcg gccctcgagc acgacaaggc ctacgaccag cagctcaagg
     2281 ccggagacaa cccgtacctc aagtacaacc acgccgacgc cgagttccag gagcggctca
     2341 aagaagatac gtcttttggg ggcaacctcg ggcgagcagt cttccaggcc aaaaagaggc
     2401 ttcttgaacc tcttggtctg gttgaggaag cggctaagac ggctcctgga aagaagaggc
     2461 ctgtagagca gtctcctcag gaaccggact cctccgcggg tattggcaaa tcgggtgcac
     2521 agcccgctaa aaagagactc aatttcggtc agactggcga cacagagtca gtcccagacc
     2581 ctcaaccaat cggagaacct cccgcagccc cctcaggtgt gggatctctt acaatggctt
     2641 caggtggtgg cgcaccagtg gcagacaata acgaaggtgc cgatggagtg ggtagttcct
     2701 cgggaaattg gcattgcgat tcccaatggc tgggggacag agtcatcacc accagcaccc
     2761 gaacctgggc cctgcccacc tacaacaatc acctctacaa gcaaatctcc aacagcacat
     2821 ctggaggatc ttcaaatgac aacgcctact tcggctacag caccccctgg gggtattttg
     2881 acttcaacag attccactgc cacttctcac cacgtgactg gcagcgactc atcaacaaca
     2941 actggggatt ccggcctaag cgactcaact tcaagctctt caacattcag gtcaaagagg
     3001 ttacggacaa caatggagtc aagaccatcg ccaataacct taccagcacg gtccaggtct
     3061 tcacggactc agactatcag ctcccgtacg tgctcgggtc ggctcacgag ggctgcctcc
     3121 cgccgttccc agcggacgtt ttcatgattc ctcagtacgg gtatctgacg cttaatgatg
     3181 gaagccaggc cgtgggtcgt tcgtcctttt actgcctgga atatttcccg tcgcaaatgc
     3241 taagaacggg taacaacttc cagttcagct acgagtttga gaacgtacct ttccatagca
     3301 gctacgctca cagccaaagc ctggaccgac taatgaatcc actcatcgac caatacttgt
     3361 actatctctc aaagactatt aacggttctg gacagaatca acaaacgcta aaattcagtg
     3421 tggccggacc cagcaacatg gctgtccagg gaagaaacta catacctgga cccagctacc
     3481 gacaacaacg tgtctcaacc actgtgactc aaaacaacaa cagcgaattt gcttggcctg
     3541 gagcttcttc ttgggctctc aatggacgta atagcttgat gaatcctgga cctgctatgg
     3601 ccagccacaa agaaggagag gaccgtttct ttcctttgtc tggatcttta atttttggca
     3661 aacaaggaac tggaagagac aacgtggatg cggacaaagt catgataacc aacgaagaag
     3721 aaattaaaac tactaacccg gtagcaacgg agtcctatgg acaagtggcc acaaaccacc
     3781 agagtgccca agcacaggcg cagaccggct gggttcaaaa ccaaggaata cttccgggta
     3841 tggtttggca ggacagagat gtgtacctgc aaggacccat ttgggccaaa attcctcaca
     3901 cggacggcaa ctttcaccct tctccgctga tgggagggtt tggaatgaaa cacccgcctc
     3961 ctcagatcct catcaaaaac acacctgtac ctgcggatcc tccaacggcc ttcaacaagg
     4021 acaagctgaa ctctttcatc acccagtatt ctactggcca agtcagcgtg gagatcgagt
     4081 gggagctgca gaaggaaaac agcaagcgct ggaacccgga gatccagtac acttccaact
     4141 attacaagtc taataatgtt gaatttgctg ttaatactga aggtgtatat agtgaacccc
     4201 gccccattgg caccagatac ctgactcgta atctgtaaac tagtgttaat caataaaccg
     4261 gttgattcgt ttcagttgaa ctttggtctc tgcgaagggc gtttaaacct gcaggactag
     4321 aggtcctgta ttagaggtca cgtgagtgtt ttgcgacatt ttgcgacacc atgtggtcac
     4381 gctgggtatt taagcccgag tgagcacgca gggtctccat tttgaagcgg gaggtttgaa
     4441 cgcgcagccg ccaagccgaa ttctgcagat atccatcaca ctggcggccg ctcgactaga
     4501 gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg
     4561 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     4621 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4681 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4741 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     4801 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4861 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4921 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4981 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5041 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5101 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5161 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5221 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5281 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5341 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5401 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5461 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5521 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5581 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5641 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5701 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5761 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5821 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5881 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5941 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6001 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     6061 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6121 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6181 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6241 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6301 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6361 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6421 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6481 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6541 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6601 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6661 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     6721 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     6781 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     6841 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     6901 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     6961 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     7021 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     7081 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     7141 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     7201 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     7261 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     7321 cgtt
//
