LOCUS       6519_pRep2-Cap8         7330 bp    DNA     circular SYN 02-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 2, 2025 from SnapGene 8.0.3
            https://www.snapgene.com
COMMENT     ApEinfo:methylated:1
            ORIGDB|GenBank
COMMENT     Sequence Label: 6519_pRep2-Cap8 (08_cap8wt)
FEATURES             Location/Qualifiers
     source          1..7330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     22..39
                     /label=7071_M13-fwd
     CDS             168..2033
                     /codon_start=1
                     /label=AAV2 Rep
                     /translation="MPGFYEIVIKVPSDLDEHLPGISDSFVNWVAEKEWELPPDSDMDL
                     NLIEQAPLTVAEKLQRDFLTEWRRVSKAPEALFFVQFEKGESYFHMHVLVETTGVKSMV
                     LGRFLSQIREKLIQRIYRGIEPTLPNWFAVTKTRNGAGGGNKVVDECYIPNYLLPKTQP
                     ELQWAWTNMEQYLSACLNLTERKRLVAQHLTHVSQTQEQNKENQNPNSDAPVIRSKTSA
                     RYMELVGWLVDKGITSEKQWIQEDQASYISFNAASNSRSQIKAALDNAGKIMSLTKTAP
                     DYLVGQQPVEDISSNRIYKILELNGYDPQYAASVFLGWATKKFGKRNTIWLFGPATTGK
                     TNIAEAIAHTVPFYGCVNWTNENFPFNDCVDKMVIWWEEGKMTAKVVESAKAILGGSKV
                     RVDQKCKSSAQIDPTPVIVTSNTNMCAVIDGNSTTFEHQQPLQDRMFKFELTRRLDHDF
                     GKVTKQEVKDFFRWAKDHVVEVEHEFYVKKGGAKKRPAPSDADISEPKRVRESVAQPST
                     SDAEASINYADRYQNKCSRHVGMNLMLFPCRQCERMNQNSNICFTHGQKDCLECFPVSE
                     SQPVSVVKKAYQKLCYIHHIMGKVPDACTACDLVNVDLDDCIFEQ"
     primer_bind     complement(203..222)
                     /label=9002_Rep78_falsch_F
     primer_bind     complement(248..265)
                     /label=18535_AAV2_Rep_Seq_R
     primer_bind     344..361
                     /label=14129_Rep_Seq_For
     primer_bind     827..846
                     /label=13265_AAV2-Rep78_Seq_For
     primer_bind     830..848
                     /label=11822_11842_GLucII_Seq_For
     primer_bind     complement(906..926)
                     /label=11843_GLucII_Seq_Rev
     primer_bind     complement(964..981)
                     /label=16153_8111_Rep_Seq_Rev
     primer_bind     968..987
                     /label=6658_Rep78_Seq-R
     primer_bind     1655..1675
                     /label=10778_Rep_Seq_F1
     primer_bind     1769..1786
                     /label=14216_Rep2_Seq_For
     primer_bind     1957..1974
                     /label=12968_Rep78end_Seq_For
     primer_bind     complement(1957..1974)
                     /label=15580_Rep78_3'_Seq_Rev
     primer_bind     1985..2004
                     /label=9003_Rep78_right_F
     primer_bind     1985..2004
                     /label=9003_Rep78_right_F (1)
     primer_bind     1991..2010
                     /label=11145_RC3_F1
     CDS             2050..4266
                     /codon_start=1
                     /label=Cap8 VP1 (WT)
                     /label=AAV\cap\8
                     /note="/vntifkey=21"
                     /translation="MAADGYLPDWLEDNLSEGIREWWALKPGAPKPKANQQKQDDGRGL
                     VLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLQAGDNPYLRYNHADAEFQERL
                     QEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTAPGKKRPVEPSPQRSPDSSTGIGKK
                     GQQPARKRLNFGQTGDSESVPDPQPLGEPPAAPSGVGPNTMAAGGGAPMADNNEGADGV
                     GSSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGATNDNTYFGYSTP
                     WGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLSFKLFNIQVKEVTQNEGTKTIANNLT
                     STIQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLE
                     YFPSQMLRTGNNFQFTYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQTTGGTA
                     NTQTLGFSQGGPNTMANQAKNWLPGPCYRQQRVSTTTGQNNNSNFAWTAGTKYHLNGRN
                     SLANPGIAMATHKDDEERFFPSNGILIFGKQNAARDNADYSDVMLTSEEEIKTTNPVAT
                     EEYGIVADNLQQQNTAPQIGTVNSQGALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSP
                     LMGGFGLKHPPPQILIKNTPVPADPPTTFNQSKLNSFITQYSTGQVSVEIEWELQKENS
                     KRWNPEIQYTSNYYKSTSVDFAVNTEGVYSEPRPIGTRYLTRNL"
     primer_bind     2349..2368
                     /label=13228_CapPKS100_qPCR_F
     primer_bind     2350..2369
                     /label=9063_P5E18-VD2/8_Seq_F1
     primer_bind     2387..2408
                     /label=9729_Seq_Cap_AAV1_F1
     primer_bind     2387..2408
                     /label=9729_Seq_Cap_AAV1_F1 (1)
     primer_bind     2457..2476
                     /label=10779_RC1_Seq_F2
     primer_bind     2459..2478
                     /label=10785_RC4_Seq_F2
     primer_bind     2477..2496
                     /label=10791_RC8_Seq_F2
     primer_bind     complement(2578..2597)
                     /label=9610_AAV8_Cap_R
     primer_bind     complement(2578..2597)
                     /label=9610_AAV8_Cap_R (1)
     primer_bind     2685..2704
                     /label=12969_Cap_Seq_For
     primer_bind     2763..2781
                     /label=13270_CapSPK100_Seq_For
     primer_bind     complement(2794..2811)
                     /label=13010_Cap8_Seq_Rev
     primer_bind     2869..2888
                     /label=11758_Seq_Primer_fwd
     primer_bind     3014..3031
                     /label=11971_11971_CombLib_For1
     primer_bind     3038..3055
                     /label=11972_CombLib_For2
     primer_bind     3040..3056
                     /label=13266_CAP8_Seq_For
     primer_bind     3096..3115
                     /label=9064_P5E18-VD2/8_Seq_F2
     primer_bind     3300..3318
                     /label=10792_RC8_Seq_F3
     primer_bind     3493..3509
                     /label=13011_Cap8_Seq_For2
     primer_bind     3633..3652
                     /label=18599_cap8NIS_qPCR_F
     primer_bind     3698..3717
                     /label=13826_AAV8_Cap_Seq_F
     primer_bind     3705..3725
                     /label=13791_AAV8_6H2_Seq_F
     primer_bind     3785..3806
                     /label=9065_P5E18-VD2/8_Seq_F3
     primer_bind     complement(3785..3806)
                     /label=11752_P5E18-VD2/8_Seq_R3
     primer_bind     complement(3977..3997)
                     /label=18600_cap8NIS_qPCR_R
     primer_bind     4112..4131
                     /label=10784_RC2_Seq_F4
     primer_bind     4112..4131
                     /label=10793_RC8_Seq_F4
     primer_bind     complement(4314..4330)
                     /label=12649_Cap_Seq_Rev
     primer_bind     4349..4370
                     /label=16154_8111_Seq_For
     primer_bind     complement(4350..4370)
                     /label=13267_Cap-3'UTR_Seq_Rev
     primer_bind     complement(4353..4373)
                     /label=11777_Seq_Primer_Rev
     primer_bind     complement(4496..4514)
                     /label=11776_Seq_Primer_Rev
     primer_bind     complement(4603..4620)
                     /label=1704_M13_rev
     primer_bind     complement(4688..4707)
                     /label=17993_JJ023
     primer_bind     complement(4752..4769)
                     /label=10464_pENTR_Seq_R
     primer_bind     complement(4922..4940)
                     /label=7521_attL_Site-Seq_Rev
     primer_bind     complement(4969..4987)
                     /label=13923_GSPT1_Seq_Rev (pMA-backbone)
     primer_bind     4991..5011
                     /label=7606_pENTR_223.1_R1
     primer_bind     complement(4997..5016)
                     /label=6639_ColEI_Rev
     primer_bind     complement(4997..5016)
                     /label=6639_ColEI_Rev (1)
     rep_origin      complement(5006..5594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication; Facilitates plasmid replication in E. coli; 
                     regulates low-copy plasmid number when Rop protein is 
                     presence (15-20) and median-copy plasmid number when Rop is
                     absent (100-300)."
     primer_bind     complement(5044..5063)
                     /label=6541_Amp_Rev_P2
     primer_bind     complement(5053..5072)
                     /label=4294_pUC_ori_Seq_R
     primer_bind     complement(5053..5072)
                     /label=10300_3746_L-ITR_F
     primer_bind     complement(5066..5084)
                     /label=14411_ori_Seq_FOR
     primer_bind     complement(5079..5100)
                     /label=11085_ITR_Seq_1
     primer_bind     5187..5206
                     /label=14154_ori_qPCR_R
     primer_bind     complement(5316..5335)
                     /label=14153_ori_qPCR_F
     variation       5447
                     /label=G->A
     primer_bind     5520..5539
                     /label=18302_Kana_Seq_for
     primer_bind     complement(5520..5539)
                     /label=4016_Col-E1_Origin_F
     primer_bind     5538..5552
                     /label=4016_Col-E1_Origin_F
     primer_bind     complement(5639..5670)
                     /label=6491_ColE1_Seq_R
     primer_bind     5663..5700
                     /label=16905_HiFi.REV
     CDS             complement(join(5765..6556,6557..6625))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5968..5987
                     /label=7632_Amp_qPCR_R
     primer_bind     complement(6101..6120)
                     /label=7631_Amp_qPCR_F
     primer_bind     6112..6130
                     /label=6542_Amp_Mitte_Seq_F
     primer_bind     complement(6209..6230)
                     /label=2473_BLAs_F2_Seq
     primer_bind     complement(6329..6353)
                     /label=15158_AmpR_Seq_F
     primer_bind     6389..6409
                     /label=6520_Amp_F_3309
     primer_bind     complement(6428..6457)
                     /label=6492_Amp_Seq_ R
     promoter        complement(6626..6730)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6661..6682
                     /label=14825_AmpProm_Seq_Rev
     primer_bind     6662..6682
                     /label=14477_Kana_Seq_R
     rep_origin      complement(6756..7211)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6820..6839)
                     /label=14155_M13 ori_qPCR_F
     primer_bind     6926..6945
                     /label=3423_F1-Ori_R
     primer_bind     complement(6926..6945)
                     /label=15222_F1_ori_F
     primer_bind     6942..6961
                     /label=14233_M13ori_qPCR_R2
     primer_bind     complement(7061..7080)
                     /label=14232_M13ori_qPCR_F2
     primer_bind     complement(7062..7082)
                     /label=14769_BAd19a_Seq_R
     primer_bind     7087..7107
                     /label=3129_F1_ori_F
     primer_bind     7144..7160
                     /label=11087_ITR_Seq_3
     primer_bind     7161..7177
                     /label=14410_F1ori_Seq_Rev
     primer_bind     7259..7278
                     /label=17994_JJ022
     primer_bind     complement(7272..7291)
                     /label=18303_Kana_Seq_rev
     primer_bind     7273..7292
                     /label=12117_P5078/79_seq_LTR_F
     primer_bind     7274..7304
                     /label=6489_RSV_Seq_F
     primer_bind     join(7322..7330,1..16)
                     /label=9961_pENTR_Geneart_Seq_F
ORIGIN
        1 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca
       61 ctatagggcg aattgggtac cgggcccccc ctcgatcgag gtcgacggta tcgggggagc
      121 tcgcagggtc tccattttga agcgggaggt ttgaacgcgc agccgccatg ccggggtttt
      181 acgagattgt gattaaggtc cccagcgacc ttgacgagca tctgcccggc atttctgaca
      241 gctttgtgaa ctgggtggcc gagaaggaat gggagttgcc gccagattct gacatggatc
      301 tgaatctgat tgagcaggca cccctgaccg tggccgagaa gctgcagcgc gactttctga
      361 cggaatggcg ccgtgtgagt aaggccccgg aggctctttt ctttgtgcaa tttgagaagg
      421 gagagagcta cttccacatg cacgtgctcg tggaaaccac cggggtgaaa tccatggttt
      481 tgggacgttt cctgagtcag attcgcgaaa aactgattca gagaatttac cgcgggatcg
      541 agccgacttt gccaaactgg ttcgcggtca caaagaccag aaatggcgcc ggaggcggga
      601 acaaggtggt ggatgagtgc tacatcccca attacttgct ccccaaaacc cagcctgagc
      661 tccagtgggc gtggactaat atggaacagt atttaagcgc ctgtttgaat ctcacggagc
      721 gtaaacggtt ggtggcgcag catctgacgc acgtgtcgca gacgcaggag cagaacaaag
      781 agaatcagaa tcccaattct gatgcgccgg tgatcagatc aaaaacttca gccaggtaca
      841 tggagctggt cgggtggctc gtggacaagg ggattacctc ggagaagcag tggatccagg
      901 aggaccaggc ctcatacatc tccttcaatg cggcctccaa ctcgcggtcc caaatcaagg
      961 ctgccttgga caatgcggga aagattatga gcctgactaa aaccgccccc gactacctgg
     1021 tgggccagca gcccgtggag gacatttcca gcaatcggat ttataaaatt ttggaactaa
     1081 acgggtacga tccccaatat gcggcttccg tctttctggg atgggccacg aaaaagttcg
     1141 gcaagaggaa caccatctgg ctgtttgggc ctgcaactac cgggaagacc aacatcgcgg
     1201 aggccatagc ccacactgtg cccttctacg ggtgcgtaaa ctggaccaat gagaactttc
     1261 ccttcaacga ctgtgtcgac aagatggtga tctggtggga ggaggggaag atgaccgcca
     1321 aggtcgtgga gtcggccaaa gccattctcg gaggaagcaa ggtgcgcgtg gaccagaaat
     1381 gcaagtcctc ggcccagata gacccgactc ccgtgatcgt cacctccaac accaacatgt
     1441 gcgccgtgat tgacgggaac tcaacgacct tcgaacacca gcagccgttg caagaccgga
     1501 tgttcaaatt tgaactcacc cgccgtctgg atcatgactt tgggaaggtc accaagcagg
     1561 aagtcaaaga ctttttccgg tgggcaaagg atcacgtggt tgaggtggag catgaattct
     1621 acgtcaaaaa gggtggagcc aagaaaagac ccgcccccag tgacgcagat ataagtgagc
     1681 ccaaacgggt gcgcgagtca gttgcgcagc catcgacgtc agacgcggaa gcttcgatca
     1741 actacgcaga caggtaccaa aacaaatgtt ctcgtcacgt gggcatgaat ctgatgctgt
     1801 ttccctgcag acaatgcgag agaatgaatc agaattcaaa tatctgcttc actcacggac
     1861 agaaagactg tttagagtgc tttcccgtgt cagaatctca acccgtttct gtcgtcaaaa
     1921 aggcgtatca gaaactgtgc tacattcatc atatcatggg aaaggtgcca gacgcttgca
     1981 ctgcctgcga tctggtcaat gtggatttgg atgactgcat ctttgaacaa taaatgattt
     2041 aaatcaggta tggctgccga tggttatctt ccagattggc tcgaggacaa cctctctgag
     2101 ggcattcgcg agtggtgggc gctgaaacct ggagccccga agcccaaagc caaccagcaa
     2161 aagcaggacg acggccgggg tctggtgctt cctggctaca agtacctcgg acccttcaac
     2221 ggactcgaca agggggagcc cgtcaacgcg gcggacgcag cggccctcga gcacgacaag
     2281 gcctacgacc agcagctgca ggcgggtgac aatccgtacc tgcggtataa ccacgccgac
     2341 gccgagtttc aggagcgtct gcaagaagat acgtcttttg ggggcaacct cgggcgagca
     2401 gtcttccagg ccaagaagcg ggttctcgaa cctctcggtc tggttgagga aggcgctaag
     2461 acggctcctg gaaagaagag accggtagag ccatcacccc agcgttctcc agactcctct
     2521 acgggcatcg gcaagaaagg ccaacagccc gccagaaaaa gactcaattt tggtcagact
     2581 ggcgactcag agtcagttcc agaccctcaa cctctcggag aacctccagc agcgccctct
     2641 ggtgtgggac ctaatacaat ggctgcaggc ggtggcgcac caatggcaga caataacgaa
     2701 ggcgccgacg gagtgggtag ttcctcggga aattggcatt gcgattccac atggctgggc
     2761 gacagagtca tcaccaccag cacccgaacc tgggccctgc ccacctacaa caaccacctc
     2821 tacaagcaaa tctccaacgg gacatcggga ggagccacca acgacaacac ctacttcggc
     2881 tacagcaccc cctgggggta ttttgacttt aacagattcc actgccactt ttcaccacgt
     2941 gactggcagc gactcatcaa caacaactgg ggattccggc ccaagagact cagcttcaag
     3001 ctcttcaaca tccaggtcaa ggaggtcacg cagaatgaag gcaccaagac catcgccaat
     3061 aacctcacca gcaccatcca ggtgtttacg gactcggagt accagctgcc gtacgttctc
     3121 ggctctgccc accagggctg cctgcctccg ttcccggcgg acgtgttcat gattccccag
     3181 tacggctacc taacactcaa caacggtagt caggccgtgg gacgctcctc cttctactgc
     3241 ctggaatact ttccttcgca gatgctgaga accggcaaca acttccagtt tacttacacc
     3301 ttcgaggacg tgcctttcca cagcagctac gcccacagcc agagcttgga ccggctgatg
     3361 aatcctctga ttgaccagta cctgtactac ttgtctcgga ctcaaacaac aggaggcacg
     3421 gcaaatacgc agactctggg cttcagccaa ggtgggccta atacaatggc caatcaggca
     3481 aagaactggc tgccaggacc ctgttaccgc caacaacgcg tctcaacgac aaccgggcaa
     3541 aacaacaata gcaactttgc ctggactgct gggaccaaat accatctgaa tggaagaaat
     3601 tcattggcta atcctggcat cgctatggca acacacaaag acgacgagga gcgttttttt
     3661 cccagtaacg ggatcctgat ttttggcaaa caaaatgctg ccagagacaa tgcggattac
     3721 agcgatgtca tgctcaccag cgaggaagaa atcaaaacca ctaaccctgt ggctacagag
     3781 gaatacggta tcgtggcaga taacttgcag cagcaaaaca cggctcctca aattggaact
     3841 gtcaacagcc agggggcctt acccggtatg gtctggcaga accgggacgt gtacctgcag
     3901 ggtcccatct gggccaagat tcctcacacg gacggcaact tccacccgtc tccgctgatg
     3961 ggcggctttg gcctgaaaca tcctccgcct cagatcctga tcaagaacac gcctgtacct
     4021 gcggatcctc cgaccacctt caaccagtca aagctgaact ctttcatcac gcaatacagc
     4081 accggacagg tcagcgtgga aattgaatgg gagctgcaga aggaaaacag caagcgctgg
     4141 aaccccgaga tccagtacac ctccaactac tacaaatcta caagtgtgga ctttgctgtt
     4201 aatacagaag gcgtgtactc tgaaccccgc cccattggca cccgttacct cacccgtaat
     4261 ctgtaaacta gtgttaatca ataaaccggt tgattcgttt cagttgaact ttggtctctg
     4321 cgaagggcgt ttaaacctgc aggactagag gtcctgtatt agaggtcacg tgagtgtttt
     4381 gcgacatttt gcgacaccat gtggtcacgc tgggtattta agcccgagtg agcacgcagg
     4441 gtctccattt tgaagcggga ggtttgaacg cgcagccgcc aagccgaatt ctgcagatat
     4501 ccatcacact ggcggccgct cgactagagc ggccgccacc gcggtggagc tccagctttt
     4561 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     4621 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     4681 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     4741 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     4801 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     4861 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     4921 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4981 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     5041 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     5101 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     5161 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     5221 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     5281 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     5341 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     5401 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     5461 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     5521 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     5581 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     5641 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     5701 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     5761 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5821 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5881 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5941 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     6001 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     6061 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     6121 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     6181 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     6241 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     6301 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     6361 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     6421 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     6481 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     6541 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     6601 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6661 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6721 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     6781 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     6841 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     6901 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     6961 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     7021 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     7081 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     7141 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     7201 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     7261 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     7321 ggtaacgcca
//
