Index of /public/2026Q1/HG002-SPRQ-Nx
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README.txt 2026-02-03 13:48 1.4K
OVERVIEW
This directory includes human HG002 WGS datasets produced
with SPRQ-Nx multi-use chemistry and two acquisitions
per SMRT Cell.
METHODS
SHEARING Pipette-tip shearing on Hamilton NGS STAR MOA 96 system
LIBRARY PREP HiFi prep kit 96 on Hamilton NGS STAR MOA 96 system
SEQUENCING Revio system, 24-hour movies
SPRQ-Nx chemistry
ANALYSIS On-instrument HiFi base and 5mCpG calling
Secondary analysis with HiFi WGS variant pipeline
RESULTS
SMRT Cell Use Movie Yield Reads Length
--------- --- ----------------------- -------- ----- -------
EA260778 1 m84009_250920_065744_s3 150.7 Gb 10.1M 14.9 kb
EA260778 2 m84009_250921_121606_s3 134.5 Gb 7.9M 17.0 kb
--------- --- ----------------------- -------- ----- -------
EA260812 1 m84009_250920_002735_s4 150.5 Gb 9.8M 15.4 kb
EA260812 2 m84009_250921_045206_s4 143.2 Gb 8.9M 16.0 kb
--------- --- ----------------------- -------- ----- -------
EA260813 1 m84009_250920_023915_s1 148.0 Gb 9.8M 15.0 kb
EA260813 2 m84009_250921_072027_s1 140.1 Gb 9.1M 15.5 kb
--------- --- ----------------------- -------- ----- -------
EA260902 1 m84009_250920_044826_s2 150.1 Gb 9.9M 15.2 kb
EA260902 2 m84009_250921_094819_s2 141.6 Gb 8.7M 16.3 kb