OVERVIEW

This directory includes a human HG002 whole genome sequencing dataset from a single SMRT Cell on the Vega system 


DIRECTORIES
    data	     		m21009_241011_231051.hifi_reads.bam
    Human-WGS-Variant-pipeline	HiFi-human-WGS-WDL_v2.0.0-rc4 and sawfish-0.12.6 output 
 

METHODS
    SHEARING         Pipette-tip shearing on the Hamilton NGS STAR MOA 96 system  
    LIBRARY PREP     HiFi prep kit 96 on Hamilton NGS STAR MOA 96
    SEQUENCING       Vega system, 24 hour movie
    ANALYSIS	     On-instrument HiFi base and 5mCpG calling

ANALYSIS

    HiFi-human-WGS-WDL workflow v2.0.0rc4 
    Haplophased bams were used to call SVs using sawfish-0.12.6